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Team:Paris Bettencourt/Experiments/YFP TetR diffusion experiments
From 2011.igem.org
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<p>More information on the design <a href="https://2011.igem.org/Team:Paris_Bettencourt/GFPLac_diffusion ">here</a>.</p> | <p>More information on the design <a href="https://2011.igem.org/Team:Paris_Bettencourt/GFPLac_diffusion ">here</a>.</p> | ||
| - | <h2> | + | <h2>Summary</h2> |
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| + | <h2>Emitter & receiver constructs in <i>B.subtilis</i> (receiver in plasmid)</h2> | ||
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| + | <h2>Emitter construct in <i>E.coli</i> - Receiver construct in <i>B.subtilis</i> (plasmid)</h2> | ||
<p>We success to integrate the TetO Array in <i>B. subtilis</i> with the multihost episomal pHM3 plasmid. <br> | <p>We success to integrate the TetO Array in <i>B. subtilis</i> with the multihost episomal pHM3 plasmid. <br> | ||
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<p>We cannot see spot apparitions specific of YFP concentration on TetO Array. This experiment leads us to think that there is no diffusion of the YFP:tetR fusion protein from <i>E. coli</i> to <i>B. subtilis</i> by nanotube.<br> | <p>We cannot see spot apparitions specific of YFP concentration on TetO Array. This experiment leads us to think that there is no diffusion of the YFP:tetR fusion protein from <i>E. coli</i> to <i>B. subtilis</i> by nanotube.<br> | ||
</p> | </p> | ||
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| + | <h2>Conclusions</h2> | ||
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Revision as of 19:54, 28 October 2011
YFP concentrator diffusion experiments
Design overview
YFP:TetR/TetO array system
More information on the design here.
Summary
Emitter & receiver constructs in B.subtilis (receiver in plasmid)
Emitter construct in E.coli - Receiver construct in B.subtilis (plasmid)
We success to integrate the TetO Array in B. subtilis with the multihost episomal pHM3 plasmid.
Experiments of E. coli (pFX234 plasmid, D. Lane strains) to B. subtilis (TetO Array, pHM3) to follow an eventual fluorescence diffusion by nanotube has been tried.
Movie : Experiment of mixed YFP:tetR (E. coli) & TetO Array (B. subtilis)
We cannot see spot apparitions specific of YFP concentration on TetO Array. This experiment leads us to think that there is no diffusion of the YFP:tetR fusion protein from E. coli to B. subtilis by nanotube.
