Revision as of 15:04, 27 October 2011

Grenoble 2011, Mercuro-Coli iGEM

Our Biobricks:

Here you can find all our news and composites parts we send to the registry.

<groupparts>iGEM011 Grenoble</groupparts>

              		
			
				
				You can also find in the registry some of our constructions intermediates:
				
				
				
				
	
		BBa_S04602
		RBS-CinR, BBa_B0034+BBa_C0077 
	
	
		BBa_S04603
		RBS-TetR, BBa_B0034+BBa_C0040 
	  
	
		BBa_S04606
		RBS-TetR-RBS-CinR, BBa_B0034+BBa_C0040+BBa_B0034+BBa_C0077
	
	
		BBa_S04605
		RBS-TetR-RBS-GFP, BBa_B0034+BBa_C0077+BBa_E0240
	
	
		BBa_S04607
		pLac-CinR, BBa_R0011+BBa_C0077
	 
	
		BBa_S04618
		pLac-CinR, BBa_K545006+BBa_I13401

MerR-del : (K545103) MerR (BBa_K346001) without RBS PCR gel Well 1: Positive control (pSB4K5) Well 2: Negative control (Without DNA) Wells 3, 4, 5, 6, 7: MerR-del (expected at 740pb, obtain around 700-800pb) Confirmed by sequencing
pMerT-GFP: (K545002) Original parts: K346002, E0240 PCR gel Well 1: MW Well 2: empty well Well 3 and 4: failed clonning Well 5: pMerT-GFP (expected at 1200pb obtain just over 1000pb) Confirmed by sequencing
pCin-CrtEBI (K545000) Originally: R0077, K274100 PCR gel Wells 1, 2 and 3: pCin-CrtEBI (Expected at 3500pb, obtain between 3000 and 4000pb) Well 4: MW Confirmed by sequencing
pConst-RBS-CinR (K545004) Originally J23119, B0034 C0077 Restriction gel Wells 1 and 2: pConst-CinR(expected at 950pb) Wells 4 and 5: failed cloning Wells 3 and 6: empty wells plasmids expected about 2 000pb, and are obtain for each well. Confirmed by sequencing
pLac-CinR (S04607) Originally R0011, E0240 Restriction gel pConst-GFP (K545001) Originally J23119, E0240 Restriction gel Well 1: pLac-CinR (expected at 950pb) Well 2: pConst-CinR (expected at 900pb) plasmids expected about 2 000pb, and are obtain for each well. Confirmed by sequencing
RBS-CinR (S04602) Originally B0034, C0077 PCR gel Well 1: RBS-CinR (expected at 1000) Wells 2, 3 and 4: failed clonning Confirmed by sequencing
RBS-TetR (S04603) Originally B0034, C0040 PCR gel well 1: RBS-TetR (expected at 950pb) Confirmed by sequencing

iGEM 2011 Main Page

@@ Line 80: / Line 80: @@
     <td>
        <p align="left">
-       <center><h5>MerR-del : K545103</h5></center><br/>
+       <center><h5>MerR-del : (K545103)</h5></center><br/><br/>
        MerR (BBa_K346001) without RBS<br/>
-       PCR gel<br/>
+       PCR gel<br/><br/>
        Well 1: Positive control (pSB4K5)<br/>
        Well 2: Negative control (Without DNA)<br/>
-       Well 3, 4, 5, 6, 7: MerR-del (expected at 740pb, obtain around 700-800pb)<br/>
+       Wells 3, 4, 5, 6, 7: MerR-del (expected at 740pb, obtain around 700-800pb)<br/><br/>
-       Confirmed by sequencing
+       Confirmed by sequencing<br/>
        </p>
     </td>
     <td>
 <center><img src="https://static.igem.org/mediawiki/2011/f/fe/01.png"/></center>
@@ Line 97: / Line 101: @@
     <td>
       <p align="left">
-      <center><h5>pMerT-GFP: K545002</h5></center><br/><br/>
+      <center><h5>pMerT-GFP: (K545002)</h5></center><br/><br/>
       Original parts: K346002, E0240<br/>
       PCR gel<br/><br/>
-      Well 1 and 2: Failed cloning<br/>
-      Well 3: pMerT-GFP (expected at1200pb obtain just over 1000pb)<br/>
+      Well 1: MW<br/>
-      Confirmed by sequencing
+     Well 2: empty well<br/>
+      Well 3 and 4: failed clonning<br/>
+     Well 5: pMerT-GFP (expected at 1200pb obtain just over 1000pb)<br/><br/>
+      Confirmed by sequencing<br/>
       </p>
     </td>
     <td>
 <center><img src="https://static.igem.org/mediawiki/2011/d/d9/02.png"/></center>
@@ Line 113: / Line 123: @@
     <td>
       <p align="left">
       <center><h5>pCin-CrtEBI (K545000)</h5></center><br/><br/>
       Originally: R0077, K274100<br/>
-      PCR gel<br/>
+      PCR gel<br/><br/>
-      Expected at 3500pb, obtain between 3000 and 4000pb<br/>
-      Confirmed by sequencing
+      Wells 1, 2 and 3: pCin-CrtEBI (Expected at 3500pb, obtain between 3000 and 4000pb)<br/>
+     Well 4: MW<br/><br/>
+      Confirmed by sequencing<br/>
     </td>
     <td>
 <center><img src="https://static.igem.org/mediawiki/2011/9/9a/03.png"/></center>
@@ Line 128: / Line 143: @@
       <p align="left">
       <center><h5>pConst-RBS-CinR (K545004)</h5></center><br/><br/>
       Originally J23119, B0034 C0077<br/>
-      Restriction gel<br/>
+      Restriction gel<br/><br/>
-      Well 1, 2: failed cloning<br/>
-      Well 3, 4: pConst-CinR(expected at 950pb)<br/>
+      Wells 1 and 2: pConst-CinR(expected at 950pb)<br/>
+     Wells 4 and 5: failed cloning<br/>
+      Wells 3 and 6: empty wells<br/>
+     plasmids expected about 2 000pb, and are obtain for each well.<br/><br/>
       Confirmed by sequencing<br/>
     </td>
     <td>
 <center><img src="https://static.igem.org/mediawiki/2011/9/96/04.png"/></center>
@@ Line 143: / Line 164: @@
       <p align="left">
       <center><h5>pLac-CinR (S04607)</h5></center><br/><br/>
       Originally R0011, E0240<br/>
-      pConst-GFP (K545001)<br/>
+      Restriction gel<br/><br/>
+     <center><h5>pConst-GFP (K545001)</h5></center><br/><br/>
       Originally J23119, E0240<br/>
-      Restriction gel<br/>
+      Restriction gel<br/><br/>
       Well 1: pLac-CinR (expected at 950pb)<br/>
       Well 2: pConst-CinR (expected at 900pb)<br/>
-      Confirmed by sequencing
+     plasmids expected about 2 000pb, and are obtain for each well.<br/><br/>
+      Confirmed by sequencing<br/>
     </td>
     <td>
 <center><img src="https://static.igem.org/mediawiki/2011/7/79/05.png"/></center>
@@ Line 160: / Line 189: @@
       <p align="left">
       <center><h5>RBS-CinR (S04602)</h5></center><br/><br/>
       Originally B0034, C0077<br/>
-      PCR gel<br/>
+      PCR gel<br/><br/>
       Well 1: RBS-CinR (expected at 1000)<br/>
-      Confirmed by sequencing
+     Wells 2, 3 and 4: failed clonning<br/><br/>
+      Confirmed by sequencing<br/>
     </td>
     <td>
 <center><img src="https://static.igem.org/mediawiki/2011/5/5f/06.png"/></center>
@@ Line 174: / Line 208: @@
       <p align="left">
       <center><h5>RBS-TetR (S04603)</h5></center><br/><br/>
       Originally B0034, C0040<br/>
-      PCR gel<br/>
+      PCR gel<br/><br/>
-      RBS-TetR (expected at 950pb)<br/>
+      well 1: RBS-TetR (expected at 950pb)<br/><br/>
       Confirmed by sequencing<br/>
     </td>
     <td>
 <center><img src="https://static.igem.org/mediawiki/2011/a/af/07.png"/></center>

Team:Grenoble/Projet/Biobricks

From 2011.igem.org

Revision as of 15:04, 27 October 2011

Our Biobricks:

MerR-del : (K545103)

pMerT-GFP: (K545002)

pCin-CrtEBI (K545000)

pConst-RBS-CinR (K545004)

pLac-CinR (S04607)

pConst-GFP (K545001)

RBS-CinR (S04602)

RBS-TetR (S04603)

Contact Us:

Clic here !

BBa_S04602	RBS-CinR, BBa_B0034+BBa_C0077
BBa_S04603	RBS-TetR, BBa_B0034+BBa_C0040
BBa_S04606	RBS-TetR-RBS-CinR, BBa_B0034+BBa_C0040+BBa_B0034+BBa_C0077
BBa_S04605	RBS-TetR-RBS-GFP, BBa_B0034+BBa_C0077+BBa_E0240
BBa_S04607	pLac-CinR, BBa_R0011+BBa_C0077
BBa_S04618	pLac-CinR, BBa_K545006+BBa_I13401