Team:ZJU-China/October.html

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<td>•Transform: Kit Plate 4 8G,8I Kit Plate 1 1F </td>
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<td>•Transform: Kit Plate 4 8G,8I Kit Plate 1 1F <br/>
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              •Culture yeast & B. subtilis</td>
                 <td>•Colony PCR and run the gel: 7M+22M+10I+1K3 1-25<br/>•Cultivate:8G,1F,8I</td>
                 <td>•Colony PCR and run the gel: 7M+22M+10I+1K3 1-25<br/>•Cultivate:8G,1F,8I</td>
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<td>•Miniprep:7M+22M+10I+1K3<br/>•Colony PCR:8I,8G,1F<br/>•Cut:7M+22M+10I+1K3,1C3 backbone</td>
<td>•Miniprep:7M+22M+10I+1K3<br/>•Colony PCR:8I,8G,1F<br/>•Cut:7M+22M+10I+1K3,1C3 backbone</td>
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                 <td>•Cultivate:7K<br/>•Ligate:7M+22M+10I& 1C3</td>
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                 <td>•Cultivate:7K<br/>•Ligate:7M+22M+10I& 1C3<br/> •Genome DNA extraction from yeast & B. subtilis<br/> •PCR condition pre-test for alsS & Adh2</td>
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<td>•Cultivate:8G,8I,1F,7M,18E,18I<br/>•Transform:7M+22M+10I+1C3 7M+22M+10I+1K3-2<br/>•Reserve:7M+22M+10I+1K3,8G,8I, 1F,7M,18E,18I</td>
<td>•Cultivate:8G,8I,1F,7M,18E,18I<br/>•Transform:7M+22M+10I+1C3 7M+22M+10I+1K3-2<br/>•Reserve:7M+22M+10I+1K3,8G,8I, 1F,7M,18E,18I</td>
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                 <td>•Miniprep:8G<br/>•Cultivate:7M+22M+10I+1C3，2J</td>
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                 <td>•Miniprep:8G<br/>•Cultivate:7M+22M+10I+1C3，2J<br/>•Testify PCR results on gel<br/>•Clean-up alsS PCR product<br/>•Re-pre-test PCR condition for Adh2</td>
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    <td > •PCR producing Adh2<br/>•alsS ES double-digestion<br/>•purification both</td>
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Revision as of 02:42, 28 October 2011

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> Notebook - September

Lab Notes

July August
 September
 October

Protocol

Protocol

Brainstorm

Brainstorm

Lab Notes - October

Biobrick Group

Week13

Day Note
Sept.26th Monday
•Miniprep: Rush-3,-4,-12
•Run the gel of digestion test results:
RFP+YFP+1K3-7,-16		 •PCR amplify: Rush-3,-4,-12
•Sample and test the intensity of fluorescence once or twice an hour during the growth of E.coli
Sept.27th Tuesday
•Ligate and transform:
RFP+YFP+CFP+1C3 RFP+YFP+1C3		 •PCR amplify:20H
•Cut: Rush Bone,22B
Sept.28th Wednesday
•Cultivate: DH5αwith Device CFP,RFP,YFP and without any device
•Ligate and purify:Rush+22B
•Cut and purify:
the product of the ligation		 •Run the gel: Digested Rush and 22B,Lig1
•Self-ligate:Digested Lig
•Colony PCR: RFP+YFP+CFP+1C3
•Cut and purify: RFP+YFP+1K3,12I
Sept.29th Thursday
•Ligate and transform: RFP+YFP+1K3&1C3,
RFP+YFP&1C3
•Colony PCR:RFP+YFP+CFP+1C3
•Preserve the colony on the plate
•Plate the ligation results: RFP+1C3,
RFP+YFP+CFP+1C3
Colony PCR:RFP+YFP+1C3
RFP+YFP+CFP+1C3
•Cultivate the above
Sept.30th Friday
•Colony PCR: Rush-Lig
Oct.1st Saturday
•Colony PCR: Failed
•MIniprep: 10I		 •Cut: 10I+22B,20J,1T3
•Ligate:10I+22B+1T3		 •Cultivate:2I,2M
Oct.2nd Sunday
•Transform the ligation results:
22B+10I(1T3),20J+10I(1T3)
•Miniprep and Preserve:2M,2I		 •Cut:fdhf,Vgb,2M,2I
•Purify:RFP+YFP+CFP+1C3		 •Ligate and transform:
RFP+YFP+CFP&1A3k
fdhf+2M&1K3,Vgb+2I&1K3

Week14

Day Note
Oct.3rd Monday
•Colony PCR
Oct.4th Tuesday
•Cut:RBS+20J
•Cultivate:fdhf+2M-1,-2,-3,-4
Vgb+2I-1,-222B+10I-1,-2
•Ligate and purify:RBS+20J& 1C3 		•Cut:RBS+20J
•Ligate:2M+20J&10I to 1K3
•Transform:2M+20J+10I,Vgb+1C3
•Cultivate:22B+10I-1,-2		 •Miniprep and cut: fdhf+2M,Vgb+2I
•Cut:fdhf+2M,20H
Oct.5th Wednesday
•Cultivate:Vgb+2I 1-6
•Ligate:fdhf+20H& 1K3 		•Transform:fdhf+20H
•Cut:2M+20J
Oct.6th Thurday
Finishing the wiki
Oct.7th Friday
making poster
Oct.8th Saturday
•Miniprep and test:
Vgb,fdhf+20H+1K3 1-4		 •Colony PCR
Oct.9th Sunday
•Digestion test and run the gel to test the results:
20J+10I -1,-2,-4
22B+10I -1,-2,-3,-4 Vgb+1C3
Fdhf+2M+20H -1,-2,-3,-4 Vgb+2I -1,-2,-5		 •Colony PCR:fdhf+2M+20H 5-14
•Cultivate:fdhf+2M+20H 5-14
•Cut:fdhf+2M,20H

 

Week15

Day Note
Oct.10th Monday
•Ligate:Fdhf+2M&20H to 1C3
•Cultivate:16L+Device C		 •Cut:Vgb+2I,22B+10I
•Ligate:Vgb+2I&22B+10I to1C3
Oct.11th Tuesday
•Cultivate:20H,22B+10I-1,20J+10I-1,-4 •Miniprep and cut:
fdhf+2M+20H+1K3 5-14
Oct.12th Wednesday
•Miniprep: fdhf+20H-10,20H,22B+10I, 20J+10I-1,-4 fdhf+2I+20H, •Cut and run the gel: Vgb+2I-5,
22B+10I,20J+10I,1C3 backbone
Oct.13th Thursday
•Cut and run the gel to test the ligate results
Oct.14th Friday
•Regional Jamboree @ HKUST
Oct.15th Saturday
•Regional Jamboree @ HKUST
Oct.16th Sunday
•Regional Jamboree @ HKUST We are the Champion!!!

 

Week16

Day Note
Oct.17th Monday
•Rest
Oct.18th Tuesday
•Rest
Oct.19th Wednesday
•Transform: Kit Plate 1 18E&18I, Kit Plate 4 7K& 7M
Oct.20th Thursday
•Transform: Kit Plate 4 7K
•Colony PCR and run the gel:23L 1-6
Oct.21st Friday
•Transform:7K
•Cultivate:7M,18I,18E,23L pSB1A3,1K3,1C3 backbone		 •Cut and test the resistance: 22M+10I
•Colony PCR:7M 1-7,18E 1-6,18I 1-6
Oct. 22nd Saturday
•Cultivate:pSB1C3-5,23L-1,-2,7M-1
•Cultivate:7K,10I,22M,18E,18I
•Colony PCR:18E 1-6,18I 1-6
•Test the reagent for PCR several times		 •Miniprep and digestion test: 7M,23L,1K3,1A3,1C3
•Digestion and run the gel: 7M,22M+10I-2,1K3
Oct.23rd Sunday
•Cut:7M,22M+10I
•Digestion test:18I,18E		 •Ligate and transform: 7M+22M+10I& 1K3

Week17

Day Note
Oct.24th Monday
•Transform: Kit Plate 4 8G,8I Kit Plate 1 1F
•Culture yeast & B. subtilis		 •Colony PCR and run the gel: 7M+22M+10I+1K3 1-25
•Cultivate:8G,1F,8I
Oct.25th Tuesday
•Miniprep:7M+22M+10I+1K3
•Colony PCR:8I,8G,1F
•Cut:7M+22M+10I+1K3,1C3 backbone		 •Cultivate:7K
•Ligate:7M+22M+10I& 1C3
•Genome DNA extraction from yeast & B. subtilis
•PCR condition pre-test for alsS & Adh2
Oct.26th Wednesday
•Cultivate:8G,8I,1F,7M,18E,18I
•Transform:7M+22M+10I+1C3 7M+22M+10I+1K3-2
•Reserve:7M+22M+10I+1K3,8G,8I, 1F,7M,18E,18I		 •Miniprep:8G
•Cultivate:7M+22M+10I+1C3，2J
•Testify PCR results on gel
•Clean-up alsS PCR product
•Re-pre-test PCR condition for Adh2
Oct.27th Thursday
•PCR producing Adh2
•alsS ES double-digestion
•purification both
Oct.28th Friday
Oct. 29th Saturday
Oct.30th Sunday


Biofilm Group

3rd Oct

4th Oct

6th Oct

12th Oct

15th Oct

16th Oct

17th Oct

20th Oct

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