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Team:Paris Liliane Bettencourt/Notebook/2011/10/22/
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==tRNA so far...== | ==tRNA so far...== | ||
===constructs=== | ===constructs=== | ||
| - | + | #S99: Phyperspank_tRNA_tt | |
| - | + | #S173: Pveg_spoVG_RFP_tt_Phs_tRNA_tt | |
| - | + | #S174: Pveg_spoVG_LacI_tt_Phs_tRNA_tt | |
| - | + | #S105: Pveg_spoVG_T7amber_tt | |
| - | + | #S136: Pveg_spoVG_GFPamber_tt | |
| - | + | #S150: double transformation of S99 (tRNA) and S136 (GFPamber) | |
| - | + | #S177: RECEIVER - pT7_spoVG_T7_spoVG_GFP_t7t_Pveg_spoVG_T7amber_tt | |
in progress: | in progress: | ||
| - | + | #sequencing of S177 | |
| - | + | #sequencing of S174 | |
| - | + | #sequencing of LacI-RFP-tRNA | |
| - | + | #completing construct: LacI-tRNA + GFPamber | |
| - | + | #completing construct: pT7_rbs_gfp_t7t + T7amber | |
===characterization=== | ===characterization=== | ||
Revision as of 03:05, 22 October 2011
tRNA so far...
constructs
- S99: Phyperspank_tRNA_tt
- S173: Pveg_spoVG_RFP_tt_Phs_tRNA_tt
- S174: Pveg_spoVG_LacI_tt_Phs_tRNA_tt
- S105: Pveg_spoVG_T7amber_tt
- S136: Pveg_spoVG_GFPamber_tt
- S150: double transformation of S99 (tRNA) and S136 (GFPamber)
- S177: RECEIVER - pT7_spoVG_T7_spoVG_GFP_t7t_Pveg_spoVG_T7amber_tt
in progress:
- sequencing of S177
- sequencing of S174
- sequencing of LacI-RFP-tRNA
- completing construct: LacI-tRNA + GFPamber
- completing construct: pT7_rbs_gfp_t7t + T7amber
characterization
tRNA + GFPamber with or without IPTG (flouorescence, microscopy, TECAN (to be updated)) T7 autoloop in BL21 with or without IPTG VS. pT7_rbs_gfp_t7t in BL21 with or without IPTG (microscopy (double check), TECAN (to be updated))
To Do: compare basal expression and IPTG induction level of tRNA + GFPamber VS. LacI-tRNA + GFPamber GFP expression with or without IPTG of (LacI)-tRNA with pT7_rbs_gfp_t7t GFP expression with or without IPTG of Receiver + Emitter
