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From 2011.igem.org

tRNA so far...

constructs

1. S99: Phyperspank_tRNA_tt
2. S173: Pveg_spoVG_RFP_tt_Phs_tRNA_tt
3. S174: Pveg_spoVG_LacI_tt_Phs_tRNA_tt
4. S105: Pveg_spoVG_T7amber_tt
5. S136: Pveg_spoVG_GFPamber_tt
6. S150: double transformation of S99 (tRNA) and S136 (GFPamber)
7. S177: RECEIVER - pT7_spoVG_T7_spoVG_GFP_t7t_Pveg_spoVG_T7amber_tt

in progress:

1. sequencing of S177
2. sequencing of S174
3. sequencing of LacI-RFP-tRNA
4. completing construct: LacI-tRNA + GFPamber
5. completing construct: pT7_rbs_gfp_t7t + T7amber

characterization

1. tRNA + GFPamber with or without IPTG (flouorescence, microscopy, TECAN (to be updated))
2. T7 autoloop in BL21 with or without IPTG VS. pT7_rbs_gfp_t7t in BL21 with or without IPTG (microscopy (double check), TECAN (to be updated))

To Do:

1. compare basal expression and IPTG induction level of tRNA + GFPamber VS. LacI-tRNA + GFPamber
2. GFP expression with or without IPTG of (LacI)-tRNA with pT7_rbs_gfp_t7t
3. GFP expression with or without IPTG of Receiver + Emitter