Team:Cornell/Week 20
From 2011.igem.org
(Difference between revisions)
Rockyshell92 (Talk | contribs) |
(→Sunday, October 16) |
||
Line 8: | Line 8: | ||
</html><br><br> | </html><br><br> | ||
==Sunday, October 16== | ==Sunday, October 16== | ||
+ | Morning lab work done by: Charlie Chung | ||
+ | :'''Preparing 100mL Cultures of VioA, B, E and pZE12 Vector''' | ||
+ | ::*Inoculate two baffled flasks of (50mL LB + 50µL ampicillin) each with 1mL of yesterday's culture for a 1:50 dilution | ||
+ | ::*Shake at 37°C for 2.5 hours and check OD reading (if 0.05-0.08, then ready for IPTG-induction) | ||
==Monday, October 17== | ==Monday, October 17== |
Revision as of 16:31, 16 October 2011
Week 1 | Week 2 - 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18 | Week 19 | Week 20 | Week 21 |
September 25th - October 1st
Sunday, October 16
Morning lab work done by: Charlie Chung
- Preparing 100mL Cultures of VioA, B, E and pZE12 Vector
- Inoculate two baffled flasks of (50mL LB + 50µL ampicillin) each with 1mL of yesterday's culture for a 1:50 dilution
- Shake at 37°C for 2.5 hours and check OD reading (if 0.05-0.08, then ready for IPTG-induction)