Team:NYMU-Taipei/results/chassis1

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Revision as of 01:05, 6 October 2011 (view source) Blackrabbit (Talk | contribs) (→AMB-1's growth curve When is the Log phase?) ← Older edit		Revision as of 01:05, 6 October 2011 (view source) Blackrabbit (Talk | contribs) (→AMB-1's growth curve When is the Log phase?) Newer edit →
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	[[Image:AMB-1growthcurve.PNG|frame|none|'''AMB-1 growth curve with MSGM.''' <br>		[[Image:AMB-1growthcurve.PNG|frame|none|'''AMB-1 growth curve with MSGM.''' <br>
-	The Horizontal axis means the time of growth and the Vertical axis means the OD565, the maximum absorbance of AMB-1. The AMB-1 enters log phase at about 7~10 days.]]	+	The Horizontal axis is the time of growth (in days) and the Vertical axis is the OD565 (the maximum absorbance of AMB-1). The AMB-1 enters log phase after about 7~10 days.]]

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Revision as of 01:05, 6 October 2011

• Home Get to HOME
• Idea Our Idea
  Concept Brainstorming Background Review
• Design/ Experiment
  Optomagnetic Design Protein Modelling NeuroSymbiosis Chassis SynthoPrime Lab Protocol
• Results
  Optomagnetic Bacteria NeuroSymbiosis Chassis Parts International Collaboration
• Human Practice & Safety
  Synthetic Neurobiology Human Practice Evaluation & Safety Assessment
• About Us
  The School The Team China Meetup Acknowledgement

AMB-1's growth curve When is the Log phase?

AMB-1 growth curve with MSGM.
The Horizontal axis is the time of growth (in days) and the Vertical axis is the OD565 (the maximum absorbance of AMB-1). The AMB-1 enters log phase after about 7~10 days.

We present AMB-1's growth curve in OD565, the maximum absorbance of AMB-1. It is important to find out the log phase, because the log phase is the best time for electroporation. Therefore, by keeping AMB-1 in "log phase" (approximately 3~6 days in Enrich-MSGM, 7~10 days in MSGM), we can get the best results for electroporation. After seven days, the bacterium would go into stationary phase. During the phase, the growth rate slows as a result of nutrient depletion and accumulation of toxic products. This phase is reached as the bacteria begin to exhaust the available resources. This phase is a constant value as the rate of bacterial growth is equal to the rate of bacterial death. So, if we want to get better quality of electroporation, we must make sure there is enough AMB-1 during log phase.