Team:Tokyo Metropolitan/Notebook/S20

From 2011.igem.org

(Difference between revisions)
(-Visualization-)
 
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Above samples
Above samples
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@restriction emzyme(XbaⅠ, PstⅠ), electrophoresis, gel extraction, electrophoresis for comfirmation, ligation oriTr4,oriTr-GFP 0A,oriTr-GFP
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@ligation pSB3C3,oriTr-GFP
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Latest revision as of 14:07, 5 October 2011

-Visualization-

-Ligation

Promoter - suppressed expression system


-Transformation

Above samples


@restriction emzyme(XbaⅠ, PstⅠ), electrophoresis, gel extraction, electrophoresis for comfirmation, ligation oriTr4,oriTr-GFP 0A,oriTr-GFP

@ligation pSB3C3,oriTr-GFP



-Targeting-

Colony PCR

Colony PCR J23100-P0140.

Electrophoresis

Electrophoresis colony PCR,but failure.

Colony PCR

Colony PCR J23100-K317037-K283030.

Electrophoresis

Electrophoresis colony PCR J23100-K317037-K283030 and find binds almost all.

Colony PCR

Colony PCR J23100-P0140.

Preculture

P0140,J23100 and J23100-K317037-K283030.