Team:UQ-Australia/Data

From 2011.igem.org

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Revision as of 11:40, 5 October 2011

A brief summary of some of our laboratory findings is included below. For more information, see the 'project' page.

We conducted a heat cycle PCR to determine the optimum conditions for amplifying our araC gene. The lanes here are loaded as follows:

1: 1kb+ ladder

2: Negative control (H2O)

3-7: pBAD33 plasmid at temperatures 50C, 55C, 58C, 60C and 65C.

8: Negative control (H2O)

9-13: pBAD33 plasmid at temperatures 50C, 55C, 58C, 60C and 65C.

The expected 900bp band for araC is seen at each temperature

PCR of the lacI gene showing the expected ~1kb band.

PCR of glnG (lanes 2 and 3), glnAp2 (lanes 5 and 6), and the promoter Plac/ara (lanes 2-4 of the second gel). Only glnG is the expected size (1.6kb), while the other two significantly smaller parts (200-300bp) have not been amplified.

@@ Line 19: / Line 19: @@
 -13: pBAD33 plasmid at temperatures 50C, 55C, 58C, 60C and 65C.
-[[File:araCpcr.png | 396x102px]]
+The expected 900bp band for araC is seen at each temperature
-PCR of the lacI gene
+[[File:AraC.jpg| 500x150px]]
-[[File:IMG_0194.JPG‎ | 231x125px]]
+PCR of the lacI gene showing the expected ~1kb band.
-PCR of glnG (lanes 2 and 3), glnAp2 (lanes 5 and 6), and the promoter Plac/ara (lanes 6-8 of the second gel). Only glnG is the expected size.
+[[File:Laci.jpg]]
-[[File:IMG_0195.JPG | 400x250px]]
+PCR of glnG (lanes 2 and 3), glnAp2 (lanes 5 and 6), and the promoter Plac/ara (lanes 2-4 of the second gel). Only glnG is the expected size (1.6kb), while the other two significantly smaller parts (200-300bp) have not been amplified.
+[[File:Glngplac.jpg‎ | 600x400px]]