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9-13: pBAD33 plasmid at temperatures 50C, 55C, 58C, 60C and 65C. | 9-13: pBAD33 plasmid at temperatures 50C, 55C, 58C, 60C and 65C. | ||
| - | [[File: | + | [[File:araCpcr.png | 396x102px]] |
PCR of the lacI gene | PCR of the lacI gene | ||
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[HERE] Summary [HERE] | 
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We conducted a heat cycle PCR to determine the optimum conditions for amplifying our araC gene. The lanes here are loaded as follows:
1: 1kb+ ladder
2: Negative control (H2O)
3-7: pBAD33 plasmid at temperatures 50C, 55C, 58C, 60C and 65C.
8: Negative control (H2O)
9-13: pBAD33 plasmid at temperatures 50C, 55C, 58C, 60C and 65C.
PCR of the lacI gene
PCR of glnG (lanes 2 and 3), glnAp2 (lanes 5 and 6), and the promoter Plac/ara (lanes 6-8 of the second gel). Only glnG is the expected size.
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