Team:UQ-Australia/Parts

From 2011.igem.org

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Revision as of 10:57, 4 October 2011

The human circadian rhythm drives many important processes in the body in accordance with the sleep/wake cycle. A characteristic of this biological clock is the periodic oscillation of gene expression. Current parts in the Registry designed to regulate periodic oscillations of gene expression have shown limited success. Here we demonstrate a biological clock being standardised as a set of BioBrick parts.
Our network is controlled by an engineered promoter, Plac/ara, which features both an activator and a repressor domain. This controls the production of downstream genes to activate other inducible promoters, pBAD and GlnAp2, eventually leading to the production of a repressor protein, lacI, which inhibits Plac/ara, resulting in oscillatory expression. This project shows the feasibility of standardising the biological clock in E. coli and grounds further development for applications in regulated drug/hormone delivery and ion channel control.

Parts

Plac/ara	125x125px A multi-domain promoter that is self-inducible by arabinose. Gene expression regulated by LacI binding to special operator sites in the promoter		glnG Encodes for DNA-binding protein(NR1), the activity of which depends on its phosphorylation. Drives expression of genes under GlnAp2 promoter
araC Encodes for a positive regulatory protein for L-arabinose utilization in E.coli		pBAD L-arabinose inducible promoter
LacI DNA-binding transcriptional inhibitor for lac-operon		GlnAp2 Promoter driven by phosphorylated GlnG(NR1)

New for iGEM 2010 is the groupparts tag. This tag will generate a table with all of the parts that your team adds to your team sandbox. Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.

<groupparts>iGEM010 UQ-Australia</groupparts>

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 |width="321"|'''<span style="color:#D4A017">Plac/ara</span>'''
+|[[File:Promoter_image_2011.png|125x125px|link=https://2011.igem.org/File:Promoter_image.png]]
 A multi-domain promoter that is self-inducible by arabinose. Gene expression regulated by LacI binding to special operator sites in the promoter
 |width="125"|