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Team:Osaka/week5
From 2011.igem.org
(Difference between revisions)
| Line 2: | Line 2: | ||
| - | ==August 28(Sun)== | + | ==August 28 (Sun)== |
# Transfer to liquid culture:09,10,11 | # Transfer to liquid culture:09,10,11 | ||
#spectrophotometry of 04 | #spectrophotometry of 04 | ||
| - | ==August 29(Mon)== | + | ==August 29 (Mon)== |
iGEM Japan Meet-Up at Kyoto University | iGEM Japan Meet-Up at Kyoto University | ||
| Line 24: | Line 24: | ||
|} | |} | ||
| - | ==August 30(Tue)== | + | ==August 30 (Tue)== |
# To liquid culture:11,3-10C | # To liquid culture:11,3-10C | ||
| - | ==August 31(Wed)== | + | ==August 31 (Wed)== |
# Miniprep of yesterday's culture | # Miniprep of yesterday's culture | ||
# Restriction digests of miniprepped parts | # Restriction digests of miniprepped parts | ||
| Line 40: | Line 40: | ||
# Transformation of ligation products | # Transformation of ligation products | ||
| - | ==September 1(Thu)== | + | ==September 1 (Thu)== |
# Transfer to liquid culture:12,13,14,15,16 | # Transfer to liquid culture:12,13,14,15,16 | ||
| - | ==September 2(Fri)== | + | ==September 2 (Fri)== |
# Miniprep of yesterday's culture | # Miniprep of yesterday's culture | ||
# Restriction digests of miniprepped parts | # Restriction digests of miniprepped parts | ||
| Line 53: | Line 53: | ||
# Transfer to liquid culture:03,04,3-10C | # Transfer to liquid culture:03,04,3-10C | ||
| - | ==September 3(Sat)== | + | ==September 3 (Sat)== |
# Miniprep of yesterday's culture | # Miniprep of yesterday's culture | ||
# Restriction digests of miniprepped parts | # Restriction digests of miniprepped parts | ||
Revision as of 13:50, 2 October 2011
Contents |
August 28 (Sun)
- Transfer to liquid culture:09,10,11
- spectrophotometry of 04
August 29 (Mon)
iGEM Japan Meet-Up at Kyoto University
- Miniprep of yesterday's culture
- Restriction digests of miniprepped parts
- Gel electrophoresis of digests
- 09 ok
- 10 ok
- 11 ×
- Transformaton of Registry parts (See Table 6).
| ID | Part Name | Resistance | Description |
|---|---|---|---|
| 3-10C | <bbpart>BBa_K115056</bbpart> | AC | IPP -> OPP or DMAPP -> OPP |
August 30 (Tue)
- To liquid culture:11,3-10C
August 31 (Wed)
- Miniprep of yesterday's culture
- Restriction digests of miniprepped parts
- Gel electrophoresis of digests
- 11,3-10C ok
- Ligation
- 12(C):1-1D(upstream)+1-2M(downstream)+1-3A(vector)
- 13(C):1-9N(upstream)+1-2M(downstream)+1-3A(vector)
- 14(A):3-10C(upstream)+2-18H(downstream)+1-5A(vector)
- 15(C):2-16N(upstream)+1-23L(downstream)+1-3A(vector)
- 16(A):3-10C(upstream)+3-6H(downstream)+1-5A(vector)
- Transformation of ligation products
September 1 (Thu)
- Transfer to liquid culture:12,13,14,15,16
September 2 (Fri)
- Miniprep of yesterday's culture
- Restriction digests of miniprepped parts
- Gel electrophoresis of digests
- 12,13,15 ok
- 14,16 ×
- Ligation
- 17(A):15(upstream)+06(downstream)+1-1G(vector)
- Transfer to liquid culture:03,04,3-10C
September 3 (Sat)
- Miniprep of yesterday's culture
- Restriction digests of miniprepped parts
- Gel electrophoresis of digests
- 3-10C × deactivation of SpeⅠ?
- Inoculation of 03,04
