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Team:Osaka/week3
From 2011.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
{{Osaka}} | {{Osaka}} | ||
| - | ==August 16(Tue)== | + | ==August 16 (Tue)== |
# Gel electrophoresis of 01,02,03,04,05,06 | # Gel electrophoresis of 01,02,03,04,05,06 | ||
# Transfer to LB culture medium:01,02,03,04,05,06,1-23L | # Transfer to LB culture medium:01,02,03,04,05,06,1-23L | ||
| Line 9: | Line 9: | ||
| - | ==August 17(Wed)== | + | ==August 17 (Wed)== |
# Miniprep of yesterday's culture | # Miniprep of yesterday's culture | ||
# Restriction digests of minipreppped parts | # Restriction digests of minipreppped parts | ||
| Line 20: | Line 20: | ||
| - | ==August 18(Thu)== | + | ==August 18 (Thu)== |
# Transfer to LB culture midium:1-3A,1-5A,1-1G,03,1-23L | # Transfer to LB culture midium:1-3A,1-5A,1-1G,03,1-23L | ||
# Transformation of 07 and Resistry parts (See Table 3) | # Transformation of 07 and Resistry parts (See Table 3) | ||
| Line 33: | Line 33: | ||
# Gel electrophoresis of digests | # Gel electrophoresis of digests | ||
| - | ==August 19(Fri)== | + | ==August 19 (Fri)== |
# Miniprep of yesterday's culture | # Miniprep of yesterday's culture | ||
# Restriction digests of minipreppped parts | # Restriction digests of minipreppped parts | ||
| Line 41: | Line 41: | ||
| - | ==August 20(Sat)== | + | ==August 20 (Sat)== |
# Gel electrophoresis of 1-5A | # Gel electrophoresis of 1-5A | ||
# Gel extraction of 1-5A | # Gel extraction of 1-5A | ||
Revision as of 13:48, 2 October 2011
Contents |
August 16 (Tue)
- Gel electrophoresis of 01,02,03,04,05,06
- Transfer to LB culture medium:01,02,03,04,05,06,1-23L
- Ligation
- 8/5 3. the same way
- Transformation of Ligation products
August 17 (Wed)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Restriction digests of 03,05
- Gel electrophoresis of digests
- Ligation
- 07(C):05(upstream)+2-16N(downstream)+1-3A(vector)
- Transfer to LB culture midium:01,02,04,05,06
August 18 (Thu)
- Transfer to LB culture midium:1-3A,1-5A,1-1G,03,1-23L
- Transformation of 07 and Resistry parts (See Table 3)
| ID | Part Name | Resistance | Description |
|---|---|---|---|
| 1-6I | <bbpart>BBa_R0040</bbpart> | A | TetR repressible promoter |
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
August 19 (Fri)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Gel extraction of 1-1G,1-3A
- Transfer to LB culture medium:1-6I,07
August 20 (Sat)
- Gel electrophoresis of 1-5A
- Gel extraction of 1-5A
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
