Team:USC/Notebook/Week9

From 2011.igem.org

(Difference between revisions)
 
Line 34: Line 34:
<table>
<table>
<tr>
<tr>
-
<h1 style="font-family:Verdana;font-weight:700;">Week 9</h1>
+
<h1 style="font-family:Verdana;font-weight:700;">Laboratory Notebook</h1>
</tr>
</tr>
Line 40: Line 40:
[[Team:USC/Notebook/Brainstorming|Brainstorming]]
[[Team:USC/Notebook/Brainstorming|Brainstorming]]
<br/>
<br/>
-
[[File:Brain_storm.JPG]]
+
[[File:Brain_storm.JPG|link=Team:USC/Notebook/Brainstorming|Brainstorming]]
</td>
</td>
Line 46: Line 46:
[[Team:USC/Notebook/Week1|Week 1]]
[[Team:USC/Notebook/Week1|Week 1]]
<br/>
<br/>
-
[[File:week1.jpg]]
+
[[File:week1.jpg|link=Team:USC/Notebook/Week1|Week 1]]
</td>
</td>
Line 52: Line 52:
[[Team:USC/Notebook/Week2|Week 2]]
[[Team:USC/Notebook/Week2|Week 2]]
<br/>
<br/>
-
[[File:week2.jpg]]
+
[[File:week2.jpg|link=Team:USC/Notebook/Week2|Week 2]]
</td>
</td>
Line 58: Line 58:
[[Team:USC/Notebook/Week3|Week 3]]
[[Team:USC/Notebook/Week3|Week 3]]
<br/>
<br/>
-
[[File:week3.jpg]]
+
[[File:week3.jpg|link=Team:USC/Notebook/Week3|Week 3]]
</td>
</td>
Line 64: Line 64:
[[Team:USC/Notebook/Week4|Week 4]]
[[Team:USC/Notebook/Week4|Week 4]]
<br/>
<br/>
-
[[File:week4.jpg]]
+
[[File:week4.jpg|link=Team:USC/Notebook/Week4|Week 4]]
</td>
</td>
Line 72: Line 72:
[[Team:USC/Notebook/Week5|Week 5]]
[[Team:USC/Notebook/Week5|Week 5]]
<br/>
<br/>
-
[[File:week5.jpg]]
+
[[File:week5.jpg|link=Team:USC/Notebook/Week5|Week 5]]
</td>
</td>
Line 78: Line 78:
[[Team:USC/Notebook/Week6|Week 6]]
[[Team:USC/Notebook/Week6|Week 6]]
<br/>
<br/>
-
[[File:week6.jpg]]
+
[[File:week6.jpg|link=Team:USC/Notebook/Week6|Week 6]]
</td>
</td>
Line 84: Line 84:
[[Team:USC/Notebook/Week7|Week 7]]
[[Team:USC/Notebook/Week7|Week 7]]
<br/>
<br/>
-
[[File:week7.jpg]]
+
[[File:week7.jpg|link=Team:USC/Notebook/Week7|Week 7]]
</td>
</td>
Line 90: Line 90:
[[Team:USC/Notebook/Week8|Week 8]]
[[Team:USC/Notebook/Week8|Week 8]]
<br/>
<br/>
-
[[File:week8.jpg]]
+
[[File:week8.jpg|link=Team:USC/Notebook/Week8|Week 8]]
</td>
</td>
Line 96: Line 96:
[[Team:USC/Notebook/Week9|Week 9]]
[[Team:USC/Notebook/Week9|Week 9]]
<br/>
<br/>
-
[[File:week9.jpg]]
+
[[File:week9.jpg|link=Team:USC/Notebook/Week9|Week 9]]
</td>
</td>
Line 104: Line 104:
[[Team:USC/Notebook/Week10|Week 10]]
[[Team:USC/Notebook/Week10|Week 10]]
<br/>
<br/>
-
[[File:week10.jpg]]
+
[[File:week10.jpg|link=Team:USC/Notebook/Week10|Week 10]]
</td>
</td>
Line 110: Line 110:
[[Team:USC/Notebook/Week11|Week 11]]
[[Team:USC/Notebook/Week11|Week 11]]
<br/>
<br/>
-
[[File:week11.jpg]]
+
[[File:week11.jpg|link=Team:USC/Notebook/Week11|Week 11]]
</td>
</td>
Line 116: Line 116:
[[Team:USC/Notebook/Week12|Week 12]]
[[Team:USC/Notebook/Week12|Week 12]]
<br/>
<br/>
-
[[File:week12.jpg]]
+
[[File:week12.jpg|link=Team:USC/Notebook/Week12|Week 12]]
</td>
</td>
Line 122: Line 122:
[[Team:USC/Notebook/Week13|Week 13]]
[[Team:USC/Notebook/Week13|Week 13]]
<br/>
<br/>
-
[[File:week13.jpg]]
+
[[File:week13.jpg|link=Team:USC/Notebook/Week13|Week 13]]
</td>
</td>
Line 128: Line 128:
[[Team:USC/Notebook/Week14|Week 14]]
[[Team:USC/Notebook/Week14|Week 14]]
<br/>
<br/>
-
[[File:week14.jpg]]
+
[[File:week14.jpg|link=Team:USC/Notebook/Week14|Week 14]]
</td>
</td>
Line 137: Line 137:
</table>
</table>
 +
<h3 style="font-family:Verdana; font-weight:700;background-color: #F0F0F0;">'''Week 9:'''</h3>
<h3 style="font-family:Verdana; font-weight:700;background-color: #F0F0F0;">'''Week 9:'''</h3>

Latest revision as of 03:57, 29 September 2011

USC Banner.jpg


Laboratory Notebook

Brainstorming
Brainstorming

Week 1
Week 1

Week 2
Week 2

Week 3
Week 3

Week 4
Week 4

Week 5
Week 5

Week 6
Week 6

Week 7
Week 7

Week 8
Week 8

Week 9
Week 9

Week 10
Week 10

Week 11
Week 11

Week 12
Week 12

Week 13
Week 13

Week 14
Week 14

Week 9:

08/01/2011
1. Lab meeting: set up all the work for the week
2. PCR CAS3
3. Make competent cells of tetR+tetO, grow overnight
4. Run digestion for PCOLA::CASO (7 samples)


08/02/2011
1. Mini prep DNA isolation of 23 plasmid
2. Digestion by BamHI and NcoI
3. Run a gel to verify
4. Prepared samples for DNA sequencing, including old ones from 07/28/2011
5. Mini-prep 48 colonies from PCOLA with CASO+4 colonies from PCDF with CASO
6. Digest 10 µL of miniprep for verification by gel


08/03/2011
1. Repeat 8 mini-prep from PCOLA with CASO
2. Digest samples from 6-8
3. PCR PCDF+CASO#4 to check if this plasmid has CASO or not
Have a positive control using 1mL of DH5α gDNA


08/04/2011
1. Make competent cells for tetR and tetO
2. Digest POCLA by NcoI-HF
3. PCR PCDF+CASO#4
Observation: gel didn’t show anything, maybe due to evaporation of PCR product, we would repeat gel later