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Team:UTP-Panama/Week 11
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==August 16== | ==August 16== | ||
===WET LAB=== | ===WET LAB=== | ||
| - | ( | + | (Wet lab morning session) |
| - | -- | + | |
| + | '''OBJECTIVE:''': Electrophoresis | ||
| + | • Preparation of 1% agarose. | ||
| + | • Wet the walls of the chamber. | ||
| + | • In a 70 mL Erlenmeyer flask was added agarose and put a minute in the microwave, then take it off shake it and put it in the microwave 20 seconds. | ||
| + | Ethidium bromide • Put in the microwave and cooled with tap water to a temperature between 60 ° C and 70 º C. | ||
| + | • Put 2.5 to 5 micolitros Ethidium bromide. We mix before pouring it into the camera. | ||
| + | • Once the gel solidified cover it with TAE (1X). (A) | ||
| + | • Add 35μL of ultra-filtered water the plasmids were at 65.5 ° C. | ||
| + | • We eliminate the droplets that were on the walls with the sping-down applied to the tubes. | ||
| + | • We put the tubes in the microwave 5 minutes. | ||
| + | • We added loading buffer which was frozen thawed How? | ||
| + | • Put dots of paper loading buffer. | ||
| + | • Of the 35 uL of plasmid take 10 mL (B). | ||
| + | • Re mix in the dots and then the wells. | ||
| + | • A and B meet. Plasmid preparation and gel electrophoresis. | ||
| + | • The plasmids with the loading buffer in the wells | ||
| + | |||
| + | o Note: It is important to place the (-) side of the DNA | ||
| + | |||
| + | • Run the gel for 3 hours. | ||
| + | • The plasmids containing épendor labeled with 1, 2, 3 are kept the cooler den -81 º C (they were 6 tubes). | ||
| + | |||
| + | o Note: We make sure that the test is running if you notice bubbles on the side of the electrodes. | ||
==August 17== | ==August 17== | ||
Revision as of 03:13, 29 September 2011
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Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | After Regional Week 1 | After Regional Week 2 | Week 11: August 15 to 20August 15WET LAB SESSIONOBJECTIVE: The results were not expected, perhaps due to the quality of cloranphenicol, for this reason we decided to prepare this antibiotic again. 1. Cloranphenicol preparation. 2. After preparing this antibiotic proceeded to prepare the control samples in 50 mL falcon tubes. 3. We use the concentration of 9.7 L of cloranphenicol by 10 mL of LB liquid. 4. We prepared two samples (control + and control -). 5. In each tube was added 5 mL of LB liquid. This LB containing an appropriate concentration of antibiotic. August 16WET LAB(Wet lab morning session) OBJECTIVE:: Electrophoresis • Preparation of 1% agarose. • Wet the walls of the chamber. • In a 70 mL Erlenmeyer flask was added agarose and put a minute in the microwave, then take it off shake it and put it in the microwave 20 seconds. Ethidium bromide • Put in the microwave and cooled with tap water to a temperature between 60 ° C and 70 º C. • Put 2.5 to 5 micolitros Ethidium bromide. We mix before pouring it into the camera. • Once the gel solidified cover it with TAE (1X). (A) • Add 35μL of ultra-filtered water the plasmids were at 65.5 ° C. • We eliminate the droplets that were on the walls with the sping-down applied to the tubes. • We put the tubes in the microwave 5 minutes. • We added loading buffer which was frozen thawed How? • Put dots of paper loading buffer. • Of the 35 uL of plasmid take 10 mL (B). • Re mix in the dots and then the wells. • A and B meet. Plasmid preparation and gel electrophoresis. • The plasmids with the loading buffer in the wells o Note: It is important to place the (-) side of the DNA • Run the gel for 3 hours. • The plasmids containing épendor labeled with 1, 2, 3 are kept the cooler den -81 º C (they were 6 tubes). o Note: We make sure that the test is running if you notice bubbles on the side of the electrodes. August 17WET LABElectrophoresis of the BBa_K381001.
August 18WET LAB(Objetives or Title): --ESCRIBIR Y MEJORAR LAB-- August 19WET LAB(Objetives or Title): --ESCRIBIR Y MEJORAR LAB-- August 20Human Practices
GENERAL SESSIONOrganization of Human Practices Project, Planning of the Next weeks in Wet lab. |
