Team:Arizona State/Notebook/September

From 2011.igem.org

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* 9/8 restriction - 9/10 ligation products transformed into NEB cells  
* 9/8 restriction - 9/10 ligation products transformed into NEB cells  
== Sunday, September 18 to Saturday, September 24 ==
== Sunday, September 18 to Saturday, September 24 ==
-
*
+
*Attempt to assemble CMR-RSR using overlapping oligos
== Sunday, September 25 to Wednesday, September 28 ==
== Sunday, September 25 to Wednesday, September 28 ==
-
*
+
*Restrict, ligate, and transform CMR and pRSF. 9/25 Send sample to sequence. 9/27 Sequencing error. They will re-run.
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Revision as of 02:28, 29 September 2011


Notebook: September


ASU Logo.png
 

Thursday, September 1

  • Started to develop Listeria Innocua project component.
  • Listeria CRISPR only uses cas9 tracr gene
  • Project requires:
  • cas9
  • Duet plasmid
  • Listeria T7 RSR
  • Ligate all together in biobrick
  • Test with protospacer

Saturday, September 3

  • 3 PCR reactions using LI as template
  • Samples visualized on gel
  • sample appears slightly higher than expected
  • some distortion and low resolution

Sunday, September 4 to Friday, September 9

  • cas9 PCR products restricted overnight

Saturday, September 10

  • 9/10 restriction products dephosphorylated for 2 hours
  • Overnight ligation of dephosphorylated restriction products

Sunday, September 11 to Saturday, September 17

  • 9/8 restriction - 9/10 ligation products transformed into NEB cells

Sunday, September 18 to Saturday, September 24

  • Attempt to assemble CMR-RSR using overlapping oligos

Sunday, September 25 to Wednesday, September 28

  • Restrict, ligate, and transform CMR and pRSF. 9/25 Send sample to sequence. 9/27 Sequencing error. They will re-run.