Team:Panama/3 September 2011
From 2011.igem.org
(Difference between revisions)
(Created page with "We repeated the PCR of the Biobrick using other master mix, with the miniprep´s products obtained from the Biobrick ordered at MIT. We prepared a chloramphenicol stock (50mg/mL)...") |
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We repeated the PCR of the Biobrick using other master mix, with the miniprep´s products obtained from the Biobrick ordered at MIT. | We repeated the PCR of the Biobrick using other master mix, with the miniprep´s products obtained from the Biobrick ordered at MIT. | ||
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We prepared a chloramphenicol stock (50mg/mL), which was stored at -20 °C. | We prepared a chloramphenicol stock (50mg/mL), which was stored at -20 °C. | ||
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Primers used for PCR: | Primers used for PCR: | ||
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• RhT2b+RhT2a | • RhT2b+RhT2a | ||
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• RhTbio2a+RhTbio2b | • RhTbio2a+RhTbio2b | ||
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• RhTbioF1b+RhTbioF1a | • RhTbioF1b+RhTbioF1a | ||
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• RhT1a+RhT1b | • RhT1a+RhT1b | ||
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Program used for PCR: | Program used for PCR: | ||
[[File:tabla2.png|700px|thumb|left|alt text]] | [[File:tabla2.png|700px|thumb|left|alt text]] |
Revision as of 00:33, 29 September 2011
We repeated the PCR of the Biobrick using other master mix, with the miniprep´s products obtained from the Biobrick ordered at MIT.
We prepared a chloramphenicol stock (50mg/mL), which was stored at -20 °C.
Primers used for PCR:
• RhT2b+RhT2a
• RhTbio2a+RhTbio2b
• RhTbioF1b+RhTbioF1a
• RhT1a+RhT1b
Program used for PCR: