Team:Panama/3 September 2011

From 2011.igem.org

(Difference between revisions)
(Created page with "We repeated the PCR of the Biobrick using other master mix, with the miniprep´s products obtained from the Biobrick ordered at MIT. We prepared a chloramphenicol stock (50mg/mL)...")
Line 1: Line 1:
We repeated the PCR of the Biobrick using other master mix, with the miniprep´s products obtained from the Biobrick ordered at MIT.
We repeated the PCR of the Biobrick using other master mix, with the miniprep´s products obtained from the Biobrick ordered at MIT.
 +
 +
We prepared a chloramphenicol stock (50mg/mL), which was stored at -20 °C.
We prepared a chloramphenicol stock (50mg/mL), which was stored at -20 °C.
 +
 +
Primers used for PCR:
Primers used for PCR:
 +
• RhT2b+RhT2a
• RhT2b+RhT2a
 +
• RhTbio2a+RhTbio2b
• RhTbio2a+RhTbio2b
 +
• RhTbioF1b+RhTbioF1a
• RhTbioF1b+RhTbioF1a
 +
• RhT1a+RhT1b
• RhT1a+RhT1b
 +
Program used for PCR:
Program used for PCR:
[[File:tabla2.png|700px|thumb|left|alt text]]
[[File:tabla2.png|700px|thumb|left|alt text]]

Revision as of 00:33, 29 September 2011

We repeated the PCR of the Biobrick using other master mix, with the miniprep´s products obtained from the Biobrick ordered at MIT.


We prepared a chloramphenicol stock (50mg/mL), which was stored at -20 °C.


Primers used for PCR:

• RhT2b+RhT2a

• RhTbio2a+RhTbio2b

• RhTbioF1b+RhTbioF1a

• RhT1a+RhT1b

Program used for PCR:

alt text