Team:UTP-Panama/Week 14

Week 14: September 5 to 10

September 5

WET LAB

Results of the Pre-experiments of the September 2 WET LAB
In these results it was not obtained any kind of fluorescence. We discarded everything used on it and reviewed possible causes of error: It’s possible that other things have grown, but they must be resistant to the chloramphenicol as the bacteria too.

Human Practices

We introduced the theoretical framework of the modeling, we put the hyperlinks whose contain the information of the biobricks.

September 6

WET LAB

Objetive :Transformation of competent cells with BBa_K381001
Protocol of transformation was carried out, to transformer competent cells with BBa_K381001. We prepared 15mL LB which were added 14.56µL of chloramphenicol, with an amount enough to prepare 3 petri dishes (2 positive controls and 1 negative control).

September 7

WET LAB

Objetive: Correction of procedures for the Experiment with BBa_K381001

1. 6 tubes
2. Set A, at 37°C:
   1. 2 tubes with 500µL KNO3 + 9.7µL chloramphenicol stock solution + BBa_K381001 (Sept 9, 2011) in 10mL LB.
   2. 1 tube for negative control without KNO3
3. Set B, at 37°C:
   1. 2 tubes with 500µL KNO3 + 9.7µL chloramphenicol stock solution + BBa_K381001 (Aug 12, 2011) in 10mL LB.
   2. 1 tube for negative control without KNO3
4. Put it in the incubator

Human Practice Meeting:

We did the drawing of renbo(renbo is the name of our project).

September 9

WET LAB

NOT LAB

September 10

General Seesion

Planinng our Scientific Comunication Activities (new name in place of outreach).