Team:UTP-Panama/Week 16
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==September 21== | ==September 21== | ||
===WET LAB=== | ===WET LAB=== | ||
- | + | ====Ligation of BBa-K381001 and BBa-K410000==== | |
- | -- | + | 1. Buffer 10X of reaction and the T4 ligase were taken out of the fridge and they are defrosted in an ice bucket. Also the process of defrosting could have been done by centrifugation. <br> |
+ | 2. Put 11µL water in a PCR tube of 200µL<br> | ||
+ | 3. Added 2µL of the sample into the tube with water<br> | ||
+ | 4. Added 2µL buffer 10X to the tube<br> | ||
+ | 5. Added 1µL T4 ligase<br> | ||
+ | 6. Mixed appropriately shaking up the tube<br> | ||
+ | 7. Incubated the reaction during 20 minutes at room temperature<br> | ||
+ | 8. Incubated the reaction during 20 minutes at 80°C. This incubation was done to deactivated the enzyme and increase the effectiveness of the transformation<br> | ||
+ | 9. Stored at -24°C in order to transform later<br> | ||
==September 22== | ==September 22== |
Revision as of 22:25, 28 September 2011
Home |
Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | After Regional Week 1 | After Regional Week 2 | Week 16: September 19 to 24September 19WET LABI. DIGESTIONFor each Eppendorf PCR was added the following: There was added the 6 components to the small tube of PCR. 7. Two (2)eppendorf tubes were incubated in the thermocycler during 1 hour at 37°C : Transformation of Competent CellsA. 4 petri dishes were filled with 5mL of solid LB 3 positive controls and 1 negative control. Total: 19.36µL para 20µL de LB 'Content of controls:' 1. In 3 eppendorf tubes was added the specified amount in positive control and were resuspended. September 20Scientific Comunication ActivitiesTree sessions: WET LABObjetives or Title: September 21WET LABLigation of BBa-K381001 and BBa-K4100001. Buffer 10X of reaction and the T4 ligase were taken out of the fridge and they are defrosted in an ice bucket. Also the process of defrosting could have been done by centrifugation. September 22WET LAB(Objetives or Title): --ESCRIBIR Y MEJORAR LAB-- September 23WET LAB(Objetives or Title): --ESCRIBIR Y MEJORAR LAB-- DNA Device Submission: Explicar September 24GENERAL SESSIONAfternoon: Talikng about WET LAB final activities. Design of the "SB UTP 1.0" and "SB UTP Project 1.0" Discussion about remake the experiments with the Bristol and Gatech Biobricks (experience). September 25WET LABSunday extra experiments session |