Team:Minnesota/leex4462-04212011000000-33

(Difference between revisions)

Latest revision as of 22:47, 27 September 2011

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Project: Surface Display

Date: Thu, 21 Apr 2011 00:00:00 GMT

Author: Sukdong Lee (leex4462)

Access: Public

Revision History:

Mon, 22 Aug 2011 10:15:27 GMT (leex4462): entry created in project'Surface Display' by leex4462 (id=3)

Purpose:

Extract the pBAD plasmid from JM109, digest the of insert and vector and purify the cut DNA.

Procedures:

-Same steps as before.

-Another liquid culture of the pBAD was inoculated due to the low yields.

Results:

Tube 2 (insert)= 21.9 ng/ul

Plasmid DNA(undigested miniprep)= 12.9


240px

@@ Line 1: / Line 1: @@
 __NOTOC__
 {{Team:Minnesota/Top}}
-<br>== Miniprep, Restriction Digest of OmpA and pucBB, and Gel extraction ==
+<br>
+== Miniprep, Restriction Digest of OmpA and pucBB, and Gel extraction ==
 '''Project:''' Surface Display
@@ Line 34: / Line 35: @@
 <nowiki>Plasmid DNA(undigested miniprep)= 12.9</nowiki>
 {{Team:Minnesota/Bottom}}