Team:Arizona State/Project/E coli

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__NOTOC__
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==Escherichia coli==
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==Experimental Design==
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This is our experimental flowchart for the E. coli CRISPR platform.
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[[Image: ASU_1655_flowchart.jpg]]
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==Plasmid Design==
[[Image: ASU_pK12CRISPR.jpg]]
[[Image: ASU_pK12CRISPR.jpg]]

Revision as of 04:58, 28 September 2011


E. coli


ASU Logo.png

Experimental Design

This is our experimental flowchart for the E. coli CRISPR platform.

ASU 1655 flowchart.jpg

Plasmid Design

ASU pK12CRISPR.jpg

The plasmid containing CRISPR derived from E. Coli was intended to include Cas genes A, B, C, D, E, and 9. In addition, it includes a leader sequence corresponding to most K12 CRISPR constructs, which is adjacent to our GFP-targeting Repeat/Spacer array.