Team:UT Dallas/ligation

From 2011.igem.org

(Difference between revisions)
Line 11: Line 11:
<script type="text/javascript">  
<script type="text/javascript">  
-
$(document).ready(function(){doCalculate()});$(function(){$('ul.llmenu').lavaLamp({speed:300,autoReturn:true,target:'li'})});$(function(){$('ul#accmenu').accordion({header:'.head',fillSpace:false,autoHeight:false,navigation:true,animated:'easeslide'})});function getNumber(o){if(!isNaN(o-0)){if(o>0)return(o-0);else return 0}else return 0}function doCalculate(){insert_vol=0;vector_vol=0;water_vol=0;p=0;Molar_Ratio_V=getNumber($('#Molar_Ratio_V').val());Molar_Ratio_I=getNumber($('#Molar_Ratio_I').val());Conc_V=getNumber($('#Conc_V').val());Conc_I=getNumber($('#Conc_I').val());Length_V=getNumber($('#Length_V').val());Length_I=getNumber($('#Length_I').val());buffer_vol=getNumber($('#buffer_vol').val());enzyme_vol=getNumber($('#enzyme_vol').val());total_vol=getNumber($('#total_vol').val());max_vol_V=getNumber($('#max_vol_V').val());max_vol_I=getNumber($('#max_vol_I').val());if((Molar_Ratio_V*Conc_I*Length_V)!=0){p=(Molar_Ratio_I*Conc_V*Length_I)/(Molar_Ratio_V*Conc_I*Length_V);if(p!=0){vector_vol=(total_vol-(buffer_vol+enzyme_vol+water_vol))/(1+p);insert_vol=total_vol-(buffer_vol+enzyme_vol+vector_vol);if(max_vol_V!=0){if(max_vol_V<vector_vol){insert_vol=(insert_vol*max_vol_V)/vector_vol;vector_vol=max_vol_V;water_vol=total_vol-(buffer_vol+enzyme_vol+vector_vol+insert_vol)}}if(max_vol_I!=0){if(max_vol_I<insert_vol){vector_vol=(vector_vol*max_vol_I)/insert_vol;insert_vol=max_vol_I;water_vol=total_vol-(buffer_vol+enzyme_vol+vector_vol+insert_vol)}}vector_vol=Math.round((total_vol-(buffer_vol+enzyme_vol+insert_vol+water_vol))*10)/10;insert_vol=Math.round((total_vol-(buffer_vol+enzyme_vol+vector_vol+water_vol))*10)/10;water_vol=Math.round((total_vol-(buffer_vol+enzyme_vol+vector_vol+insert_vol))*10)/10;$('#buffer_vol1').html(buffer_vol);$('#enzyme_vol1').html(enzyme_vol);$('#total_vol1').html(total_vol);$('#vector_vol').html(vector_vol);$('#insert_vol').html(insert_vol);$('#water_vol').html(water_vol)}}}
+
$(document).ready(function(){doCalculate()});$(function(){
 +
$('ul.llmenu').find("a[href='https://2011.igem.org/Team:UT_Dallas/test']").parent().addClass('selectedLava');
 +
        $('ul#accmenu').accordion({
 +
              header:'.head',
 +
              fillSpace:false,
 +
              autoHeight:false,
 +
              navigation:true,
 +
              animated: 'easeslide'});
 +
$('ul.llmenu').lavaLamp({ speed: 300, autoReturn: true , target:'li'});
 +
});function getNumber(o){if(!isNaN(o-0)){if(o>0)return(o-0);else return 0}else return 0}function doCalculate(){insert_vol=0;vector_vol=0;water_vol=0;p=0;Molar_Ratio_V=getNumber($('#Molar_Ratio_V').val());Molar_Ratio_I=getNumber($('#Molar_Ratio_I').val());Conc_V=getNumber($('#Conc_V').val());Conc_I=getNumber($('#Conc_I').val());Length_V=getNumber($('#Length_V').val());Length_I=getNumber($('#Length_I').val());buffer_vol=getNumber($('#buffer_vol').val());enzyme_vol=getNumber($('#enzyme_vol').val());total_vol=getNumber($('#total_vol').val());max_vol_V=getNumber($('#max_vol_V').val());max_vol_I=getNumber($('#max_vol_I').val());if((Molar_Ratio_V*Conc_I*Length_V)!=0){p=(Molar_Ratio_I*Conc_V*Length_I)/(Molar_Ratio_V*Conc_I*Length_V);if(p!=0){vector_vol=(total_vol-(buffer_vol+enzyme_vol+water_vol))/(1+p);insert_vol=total_vol-(buffer_vol+enzyme_vol+vector_vol);if(max_vol_V!=0){if(max_vol_V<vector_vol){insert_vol=(insert_vol*max_vol_V)/vector_vol;vector_vol=max_vol_V;water_vol=total_vol-(buffer_vol+enzyme_vol+vector_vol+insert_vol)}}if(max_vol_I!=0){if(max_vol_I<insert_vol){vector_vol=(vector_vol*max_vol_I)/insert_vol;insert_vol=max_vol_I;water_vol=total_vol-(buffer_vol+enzyme_vol+vector_vol+insert_vol)}}vector_vol=Math.round((total_vol-(buffer_vol+enzyme_vol+insert_vol+water_vol))*10)/10;insert_vol=Math.round((total_vol-(buffer_vol+enzyme_vol+vector_vol+water_vol))*10)/10;water_vol=Math.round((total_vol-(buffer_vol+enzyme_vol+vector_vol+insert_vol))*10)/10;$('#buffer_vol1').html(buffer_vol);$('#enzyme_vol1').html(enzyme_vol);$('#total_vol1').html(total_vol);$('#vector_vol').html(vector_vol);$('#insert_vol').html(insert_vol);$('#water_vol').html(water_vol)}}}
</script>
</script>
Line 66: Line 75:
<div id="container2">
<div id="container2">
<div id="container1">
<div id="container1">
-
<div id="left">  
+
<div id="left">
-
<ul id="accmenu">
+
</html>{{:Team:UT_Dallas/Template:menu}}<html>
-
<li><a class="head" href="#"> Introduction</a>
+
-
<ul class="llmenu llmenublue">
+
-
<li><a href="https://2011.igem.org/Team:UT_Dallas/test">Home</a></li>
+
-
<li><a href="#">Team</a></li>
+
-
<li><a href="https://2011.igem.org/Team:UT_Dallas/gallery">Gallery</a></li>
+
-
<li><a href="#">Parts submitted</a></li>
+
-
</ul>
+
-
</li>
+
-
<li><a class="head" href="#">Sensor</a>
+
-
<ul class="llmenu llmenugreen">
+
-
<li><a href="#">Introduction</a></li>
+
-
<li><a href="#">Overview</a></li>
+
-
<li><a href="#">Plan</a></li>
+
-
<li><a href="#">Characterization</a></li>
+
-
</ul>
+
-
</li>
+
-
 
+
-
<li><a class="head" href="#">Taxis</a>
+
-
<ul class="llmenu llmenublue">
+
-
<li><a href="#">Introduction</a></li>
+
-
<li><a href="#">Overview</a></li>
+
-
<li><a href="#">Plan</a></li>
+
-
<li><a href="#">Characterization</a></li>
+
-
</ul>
+
-
</li>
+
-
+
-
<li><a class="head" href="#">Inactivate</a>
+
-
<ul class="llmenu llmenublue">
+
-
<li><a href="#">Introduction</a></li>
+
-
<li><a href="#">Overview</a></li>
+
-
<li><a href="#">Plan</a></li>
+
-
<li><a href="#">Characterization</a></li>
+
-
</ul>
+
-
</li>
+
-
+
-
<li><a class="head" href="#">Toolbox</a>
+
-
<ul class="llmenu llmenublue">
+
-
<li class="selectedLava"><a href="https://2011.igem.org/Team:UT_Dallas/ligation">Ligation Calculator</a></li>
+
-
<li><a href="https://2011.igem.org/Team:UT_Dallas/NEB_double_digest">NEB Double Digest</a></li>
+
-
<li><a href="#">Solutions Maker</a></li>
+
-
</ul>
+
-
</li>
+
-
+
-
 
+
-
<li><a class="head" href="#">Considerations</a>
+
-
<ul class="llmenu llmenublue">
+
-
<li><a href="#">Human Practices</a></li>
+
-
<li><a href="#">Safety</a></li>
+
-
</ul>
+
-
</li>
+
-
 
+
-
<li><a class="head" href="#">Future applications</a>
+
-
 
+
-
<ul class="llmenu llmenured">
+
-
<li><a href="#">TBA</a></li>
+
-
</ul>
+
-
</li>
+
-
 
+
-
 
+
-
<li><a class="head" href="#/Notebook/Week1">Notebook</a>
+
-
<ul class="llmenu llmenuyellow">
+
-
<li><a href="#/Notebook/Summary">Summary</a></li>
+
-
<li><a href="#/Notebook/Week1">Week 1</a></li>
+
-
<li><a href="#/Notebook/Week2">Week 2</a></li>
+
-
 
+
-
<li><a href="#/Notebook/Week3">Week 3</a></li>
+
-
<li><a href="#/Notebook/Week4">Week 4</a></li>
+
-
<li><a href="#/Notebook/Week5">Week 5</a></li>
+
-
<li><a href="#/Notebook/Week6">Week 6</a></li>
+
-
<li><a href="#/Notebook/Week7">Week 7</a></li>
+
-
<li><a href="#/Notebook/Week8">Week 8</a></li>
+
-
<li><a href="#/Notebook/Week9">Week 9</a></li>
+
-
<li><a href="#/Notebook/Week10">Week 10</a></li>
+
-
<li><a href="#/Notebook/Week11">Week 11</a></li>
+
-
 
+
-
<li><a href="#/Notebook/Week12">Week 12</a></li>
+
-
<li><a href="#/Notebook/FurtherWork">Beyond Week 12</a></li>
+
-
 
+
-
<li><a href="https://2011.igem.org/Team:UT_Dallas/protocols_new">Protocols</a> </li>
+
-
</ul>
+
-
</li>
+
-
<li><a href="#" class="head">Our Partners</a>
+
-
<ul class="llmenu llmenublue">
+
-
<li><a href="#">Sponsors</a> </li>
+
-
<li><a href="#">UT Dallas</a> </li>
+
-
<li><a href="#">Publicity</a> </li>
+
-
 
+
-
</ul> </li>
+
-
 
+
-
</ul>
+
</div>
</div>
<div id="right"> <div id="app" style="float:right">
<div id="right"> <div id="app" style="float:right">

Revision as of 06:07, 28 September 2011

Retrieved from "http://2011.igem.org/Team:UT_Dallas/ligation"