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Team:UT Dallas/ligation
From 2011.igem.org
Ligation Calculator
| Total Reaction Mix(µl) | ||
| Volume of the buffer(µl) | ||
| Volume of the enzyme(µl) | ||
| Vector | Insert | |
| Molar Ratio | ||
| Concentration(ng/µl) | ||
| Length(bp) | ||
| Maximum volume(µl) | ||
| Known Vector Lengths | ||
Ligation Mix
| Vol. in µl | |
| Buffer | 1 |
| Enzyme | 1 |
| Vector | 0 |
| Insert | 0 |
| Water | 0 |
| Total | 10 |
