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Team:Harvard/Technology
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=Zinc Finger Binding Site Finder= | =Zinc Finger Binding Site Finder= | ||
Check out our Zinc Finger Binding Site Finder Tool [[Team:Harvard/ZF_Binding_Site_Finder|here]]! This tool was used to search the human genome for the six target DNA sequences that we used to design our custom zinc finger arrays. | Check out our Zinc Finger Binding Site Finder Tool [[Team:Harvard/ZF_Binding_Site_Finder|here]]! This tool was used to search the human genome for the six target DNA sequences that we used to design our custom zinc finger arrays. | ||
Revision as of 05:22, 27 September 2011
Overview | MAGE | Chip-Based Synthesis | Lambda Red | Protocols
Our project uses 3 major new technologies: Multiplex Automated Genome Engineering (MAGE), chip-based synthesis of DNA, and lamba red recombination, along with more traditional bioinformatics. We hope that future iGEM teams will also use these techniques in their own synthetic biology projects.
Bioinformatics
See our bioinformatics page for details on the computer aspects of our technology.
Zinc Finger Binding Site Finder
Check out our Zinc Finger Binding Site Finder Tool here! This tool was used to search the human genome for the six target DNA sequences that we used to design our custom zinc finger arrays.
Lambda Red- Mediated Recombineering
Useful Links
- Gene Knockouts and Exchanges by Linear Transformation: http://rothlab.ucdavis.edu/protocols/Lin.Transform.html
- Open Wet Ware Protocol: http://openwetware.org/wiki/Recombineering/Lambda_red-mediated_gene_replacement
Gibson (Isothermal) Assembly
Summary (Adapted from Gibson et al)4,5
- Source: http://www.nature.com/nmeth/journal/v6/n5/full/nmeth.1318.html
- Protocol: http://www.nature.com/protocolexchange/protocols/554#/main
References
4. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA 3rd, Smith HO. (2009). Enzymatic assembly of DNA molecules up to several hundred kilobases. Nature Methods,6(5):343-5. Epub 2009 Apr 12. [http://www.nature.com/nmeth/journal/v6/n5/full/nmeth.1318.html]
5. Gibson D. (2009). One-step enzymatic assembly of DNA molecules up to several hundred kilobases in size. Nature Protocols,Published online 16 April 2009, doi:10.1038/nprot.2009.77.
[http://dx.doi.org/10.1038/nprot.2009.77]
