Team:Arizona State/Lab/Protocols/Gel visualization

# Mix 1x TAE Buffer with 0.50g agarose in 250 mL Erlenmeyer flask. Swirl well.  

# Microwave solution until it begins to boil. Gently swirl the flask and repeat the microwaving process until the agarose is completely dissolved and the solution is clear.

# Add 5 μL dye (SYBR green or ethidium bromide) and gently swirl the mixture. Let the solution sit for 5 minutes.

# Carefully pour the solution into the 50 mL gel tray and add in the desired comb. Let sit for 30-45 minutes until gel solidifies.  

# Place gel tray in the -4C refrigerator for 10-15 minutes.  

# Remove gel tray from refrigerator, remove rubber ends, and place the gel in electrophoresis chamber with the comb towards the negative end.  

# Add in enough 1x TAE Buffer in the chamber to just barely create a thin film of buffer over the gel.

# Add 2 μL of each DNA sample to a strip of parafilm. Add 8 μL of loading buffer to each of those 2 μL samples.  

# Add the necessary DNA ladder to the first well in the gel.

# Add each 10 μL mix of buffer and DNA into individual wells in the gel.

# Attach the electrophoresis chamber lid onto the chamber and plug into the power source.  

#Turn off the power source, remove the gel, and use ultraviolet light to visualize the DNA bands.