Team:Arizona State/Lab/Protocols/PCR

(Difference between revisions)

Latest revision as of 23:01, 26 September 2011

	blank	0	1	2	4	8	10
MgCl2	0	0	.5	1	2	4	5
DNA	0	1	1	1	1	1	1

@@ Line 1: / Line 1: @@
 {{:Team:Arizona State/Templates/main|title=[[Team:Arizona State/Lab/Protocols|Protocols]]: PCR|content=
 &nbsp;
-'''PCR:'''
-----
-* Prepare master mix (for 8 tubes):
-* 10*n uL 5x Phusion buffer
-* 4*n uL 2.5 mM dNTPs
-* 2.5*n uL forward primer
-* 2.5*n uL reverse primer
-* 29.5*n uL PCR water
-* Add 48 uL master mix to each of seven tubes.
-* Label tubes blank, 0, 1, 2, 4, 8, 10
-* Add DNA and MgCl2 to tubes according to chart:
-<!-- it appears that tables don't work due to the piping of content-->
-Blank 0 1 2 4 8 10<br>
-MgCl2 0 0 .5 1 2 4 5<br>
-DNA   0 1 1 1 1 1 1<br>
-* Add 0.5 uL Phusion polymerase to each tube.
+# For 50 ul reactions, prepare master mix as follows for '''n''' tubes:
-* Place tubes in thermocycler.
+#: 10*n uL 5x Phusion buffer
-* Adjust settings as needed.
+#: 4*n uL 2.5 mM dNTPs
+#: 2.5*n uL forward primer
+#: 2.5*n uL reverse primer
+#: 29.5*n uL PCR water
+# Add 48 uL master mix to each of seven tubes.
+# Label tubes blank, 0, 1, 2, 4, 8, 10
+# Add DNA and MgCl2 to tubes according to chart:
+<center>{{:Team:Arizona State/Templates/PCR table}}</center>
+# Add 0.5 uL Phusion polymerase to each tube.
+# Place tubes in thermocycler.
+# Adjust settings as needed.
 }}