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Team:Arizona State/Notebook/September
From 2011.igem.org
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== Thursday, September 1 == | == Thursday, September 1 == | ||
| - | + | * Started to develop Listeria Innocua project component. | |
| + | :* Listeria CRISPR only uses cas9 tracr gene | ||
| + | :* Project requires: | ||
| + | ::* cas9 | ||
| + | ::* Duet plasmid | ||
| + | ::* Listeria T7 RSR | ||
| + | ::* Ligate all together in biobrick | ||
| + | ::* Test with protospacer | ||
== Saturday, September 3 == | == Saturday, September 3 == | ||
| + | * 3 PCR reactions using LI as template | ||
| + | * Samples visualized on gel | ||
| + | :* sample appears slightly higher than expected | ||
| + | ::* some distortion and low resolution | ||
== Sunday, September 4 == | == Sunday, September 4 == | ||
== Monday, September 5 == | == Monday, September 5 == | ||
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== Thursday, September 8 == | == Thursday, September 8 == | ||
== Friday, September 9 == | == Friday, September 9 == | ||
| + | * cas9 PCR products restricted overnight | ||
== Saturday, September 10 == | == Saturday, September 10 == | ||
| + | * 9/10 restriction products dephosphorylated for 2 hours | ||
| + | * Overnight ligation of dephosphorylated restriction products | ||
== Sunday, September 11 == | == Sunday, September 11 == | ||
| + | * 9/8 restriction - 9/10 ligation products transformed into NEB cells | ||
== Monday, September 12 == | == Monday, September 12 == | ||
== Tuesday, September 13 == | == Tuesday, September 13 == | ||
| + | * | ||
== Wednesday, September 14 == | == Wednesday, September 14 == | ||
== Thursday, September 15 == | == Thursday, September 15 == | ||
Revision as of 08:05, 28 September 2011
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 Thursday, September 1
Saturday, September 3
Sunday, September 4Monday, September 5Tuesday, September 6Wednesday, September 7Thursday, September 8Friday, September 9
Saturday, September 10
Sunday, September 11
Monday, September 12Tuesday, September 13Wednesday, September 14Thursday, September 15Friday, September 16Saturday, September 17Sunday, September 18Monday, September 19Tuesday, September 20Wednesday, September 21Thursday, September 22Friday, September 23Saturday, September 24Sunday, September 25Monday, September 26Tuesday, September 27== Wednesday, September 28 == |
