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Team:Harvard/Project/Zinc Finger Background
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ZFPs consist of smaller subunits called "fingers" which each contain a zinc finger binding helix that binds to unique DNA sequences. These fingers are linear and linked together by the "zinc finger backbone", a series of approximately 21 amino acids. | ZFPs consist of smaller subunits called "fingers" which each contain a zinc finger binding helix that binds to unique DNA sequences. These fingers are linear and linked together by the "zinc finger backbone", a series of approximately 21 amino acids. | ||
| - | Cis2His2 ZFPs have three main structural components: | + | *Cis2His2 ZFPs have three main structural components: |
| - | + | ** Zinc finger binding helix | |
| - | + | ** Linker region | |
| - | + | ** Zinc ion that is coordinated by two cysteine residues and two histidine residues. | |
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Revision as of 16:51, 25 September 2011
Overview | Bioinformatics | Chip-Based Library | Plasmid Construction | Selection Strain Engineering | Protocols
What are Zinc Finger Proteins (ZFPs)?
Function
ZFPs are found commonly in nature as a class of special transcription factors that bind to DNA, thus regulating gene expression.
Structure
ZFPs consist of smaller subunits called "fingers" which each contain a zinc finger binding helix that binds to unique DNA sequences. These fingers are linear and linked together by the "zinc finger backbone", a series of approximately 21 amino acids.
- Cis2His2 ZFPs have three main structural components:
- Zinc finger binding helix
- Linker region
- Zinc ion that is coordinated by two cysteine residues and two histidine residues.
