Team:USC/Notebook/Week4

From 2011.igem.org

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                        <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Attributions|Attributions]]</span></span>
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<h1 style="font-family:Verdana;font-weight:700;">Week 4</h1>
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[[Team:USC/Notebook/Week14|Week 14]]
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<h3 style="font-family:Verdana; font-weight:700;background-color: #F0F0F0;">'''Week 4:'''</h3>
<h3 style="font-family:Verdana; font-weight:700;background-color: #F0F0F0;">'''Week 4:'''</h3>
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Observation: tetO didn’t work, OLE1 and MSN2 worked
Observation: tetO didn’t work, OLE1 and MSN2 worked
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Revision as of 03:22, 29 September 2011

USC Banner.jpg


Week 4

Brainstorming
Brain storm.JPG

Week 1
Week1.jpg

Week 2
Week2.jpg

Week 3
Week3.jpg

Week 4
Week4.jpg

Week 5
Week5.jpg

Week 6
Week6.jpg

Week 7
Week7.jpg

Week 8
Week8.jpg

Week 9
Week9.jpg

Week 10
Week10.jpg

Week 11
Week11.jpg

Week 12
Week12.jpg

Week 13
Week13.jpg

Week 14
Week14.jpg

Week 4:

06/27/2011
1. Lab meeting


06/28/2011
1. Observation from yesterday:
Only transformation of OLE1grow on plates, others don’t
2. PCR MSN ligation products in Cyle #1, pick E, F, G , H colony
3. Ligate OLE1, MSN2, TSP1, ELO1 with pRS vectors 423-426
4. Transform the ligation samples
5. Plasmid DNA purification for:
BBa-K191005
BBa-K191004
BBa-K426020
BBa-I15010


06/30/2011
1. Digest MET25 and pRS vectors
Notes: for pRS vectors, add 1 µL of phosphatase, incubate for 30min and spin column purify
For MET25 promoter, run reaction on a1% agarose gel, then cut out the agarose and purify with column
2. Transform and inoculate tetR, tetO, CASO and CRISPR


07/01/2011
1. Mini-prep
tetR, MET25, CRISPR and tetO
2. Gel verification for
TPS1, MSN2, ELO1, OLE1, tetO, MET25, CRISPR, tetR
Observation: tetO didn’t work, OLE1 and MSN2 worked

Retrieved from "http://2011.igem.org/Team:USC/Notebook/Week4"