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Team:USC/Notebook/Week7
From 2011.igem.org
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<div style = "background: rgb(153,0,0);width: 954px;height: 36px;margin: 0 auto;padding: 0;"> | <div style = "background: rgb(153,0,0);width: 954px;height: 36px;margin: 0 auto;padding: 0;"> | ||
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| - | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC|Home]]</span></span> |
| - | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Team|Team]]</span></span> |
| - | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Project|Project]]</span></span> |
| - | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Notebook|Notebook]]</span></span> | |
| - | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Parts|Parts]]</span></span> |
| - | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Safety|Safety]]</span></span> |
| - | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Human Outreach|Human Outreach]]</span></span> |
| - | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Attributions|Attributions]]</span></span> |
| + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[https://igem.org/Team.cgi?year=2011&team_name=USC Official Team Profile]</span></span> | ||
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| + | <h1 style="font-family:Verdana;font-weight:700;">Brainstorming</h1> | ||
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| - | [[Team:USC/Notebook/Week13|Week 13 | + | [[Team:USC/Notebook/Week13|Week 13]] |
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[[File:week13.jpg]] | [[File:week13.jpg]] | ||
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| + | [[Team:USC/Notebook/Week14|Week 14]] | ||
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<h3 style="font-family:Verdana; font-weight:700;background-color: #F0F0F0;">'''Week 7:'''</h3> | <h3 style="font-family:Verdana; font-weight:700;background-color: #F0F0F0;">'''Week 7:'''</h3> | ||
Revision as of 03:35, 29 September 2011
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Week 7:
07/19/2011
1. Gel verification for CASO (1-8)
Observation: in second row, GFP spcer, tetR spacer, CRISPR#1, and CRISPR #2
2. Gel verification by digestion
3. Purify tetR from Gel
4. Ligate pSB1C3 with tetR, place in the 15c incubator overnight
5. Transformation
07/21/2011
1. Transform ligations of tetR in DH5α and top10 competent cells
2. Inoculate PCOLA, PCDF and CRISPR
07/22/2011
1. Mini-prep for :
tetR in DH5α and top10 (16 samples)
CRISPR (2 samples)
PCOLA (1 sample)
PCDF (5 samples)
2. Digest tetR with EcoR1 and Pst1
3. Test the digested tetR by running a gel
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