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Team:USC/Notebook/Week9
From 2011.igem.org
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<div style = "background: rgb(153,0,0);width: 954px;height: 36px;margin: 0 auto;padding: 0;"> | <div style = "background: rgb(153,0,0);width: 954px;height: 36px;margin: 0 auto;padding: 0;"> | ||
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| - | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC|Home]]</span></span> |
| - | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Team|Team]]</span></span> |
| - | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Project|Project]]</span></span> |
| - | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Notebook|Notebook]]</span></span> | |
| - | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Parts|Parts]]</span></span> |
| - | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Safety|Safety]]</span></span> |
| - | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Human Outreach|Human Outreach]]</span></span> |
| - | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: | + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[[Team:USC/Attributions|Attributions]]</span></span> |
| + | <span style="float: left; padding: 15px;"><span style="font-family: Arial, Helvetica, sans-serif;font-size: 15px;font-weight: bold;color: #FFFFFF;border: none;">[https://igem.org/Team.cgi?year=2011&team_name=USC Official Team Profile]</span></span> | ||
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| + | <h1 style="font-family:Verdana;font-weight:700;">Week 9</h1> | ||
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| - | [[Team:USC/Notebook/Week13|Week 13 | + | [[Team:USC/Notebook/Week13|Week 13]] |
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[[File:week13.jpg]] | [[File:week13.jpg]] | ||
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| + | [[Team:USC/Notebook/Week14|Week 14]] | ||
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<h3 style="font-family:Verdana; font-weight:700;background-color: #F0F0F0;">'''Week 9:'''</h3> | <h3 style="font-family:Verdana; font-weight:700;background-color: #F0F0F0;">'''Week 9:'''</h3> | ||
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Observation: gel didn’t show anything, maybe due to evaporation of PCR product, we would repeat gel later | Observation: gel didn’t show anything, maybe due to evaporation of PCR product, we would repeat gel later | ||
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Revision as of 03:40, 29 September 2011
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Week 9:
08/01/2011
1. Lab meeting: set up all the work for the week
2. PCR CAS3
3. Make competent cells of tetR+tetO, grow overnight
4. Run digestion for PCOLA::CASO (7 samples)
08/02/2011
1. Mini prep DNA isolation of 23 plasmid
2. Digestion by BamHI and NcoI
3. Run a gel to verify
4. Prepared samples for DNA sequencing, including old ones from 07/28/2011
5. Mini-prep 48 colonies from PCOLA with CASO+4 colonies from PCDF with CASO
6. Digest 10 µL of miniprep for verification by gel
08/03/2011
1. Repeat 8 mini-prep from PCOLA with CASO
2. Digest samples from 6-8
3. PCR PCDF+CASO#4 to check if this plasmid has CASO or not
Have a positive control using 1mL of DH5α gDNA
08/04/2011
1. Make competent cells for tetR and tetO
2. Digest POCLA by NcoI-HF
3. PCR PCDF+CASO#4
Observation: gel didn’t show anything, maybe due to evaporation of PCR product, we would repeat gel later
