Team:USC/Notebook/Week7

From 2011.igem.org

(Difference between revisions)

Revision as of 05:59, 25 September 2011

Official Team Profile

Week 7

Week 7:

07/19/2011
1. Gel verification for CASO (1-8)
Observation: in second row, GFP spcer, tetR spacer, CRISPR#1, and CRISPR #2
2. Gel verification by digestion
3. Purify tetR from Gel
4. Ligate pSB1C3 with tetR, place in the 15c incubator overnight
5. Transformation

07/21/2011
1. Transform ligations of tetR in DH5α and top10 competent cells
2. Inoculate PCOLA, PCDF and CRISPR

07/22/2011
1. Mini-prep for :
tetR in DH5α and top10 (16 samples)
CRISPR (2 samples)
PCOLA (1 sample)
PCDF (5 samples)
2. Digest tetR with EcoR1 and Pst1
3. Test the digested tetR by running a gel

@@ Line 50: / Line 50: @@
 <td style="text-align:center; font-family:Verdana; font-weight:700;">
-[[Team:USC/Notebook/Brainstorming|Week 1]]
+[[Team:USC/Notebook/Week1|Week 1]]
 <br/>
 [[File:week1.jpg]]
@@ Line 58: / Line 58: @@
 <td style="text-align:center; font-family:Verdana; font-weight:700;">
-[[Team:USC/Notebook/Brainstorming|Week 2]]
+[[Team:USC/Notebook/Week2|Week 2]]
 <br/>
 [[File:week2.jpg]]
@@ Line 64: / Line 64: @@
 <td style="text-align:center; font-family:Verdana; font-weight:700;">
-[[Team:USC/Notebook/Brainstorming|Week 3]]
+[[Team:USC/Notebook/Week3|Week 3]]
 <br/>
 [[File:week3.jpg]]
@@ Line 70: / Line 70: @@
 <td style="text-align:center; font-family:Verdana; font-weight:700;">
-[[Team:USC/Notebook/Brainstorming|Week 4]]
+[[Team:USC/Notebook/Week4|Week 4]]
 <br/>
 [[File:week4.jpg]]
@@ Line 76: / Line 76: @@
 <td style="text-align:center; font-family:Verdana; font-weight:700;">
-[[Team:USC/Notebook/Brainstorming|Week 5]]
+[[Team:USC/Notebook/Week5|Week 5]]
 <br/>
 [[File:week5.jpg]]
@@ Line 82: / Line 82: @@
 <td style="text-align:center; font-family:Verdana; font-weight:700;">
-[[Team:USC/Notebook/Brainstorming|Week 6]]
+[[Team:USC/Notebook/Week6|Week 6]]
 <br/>
 [[File:week6.jpg]]
@@ Line 90: / Line 90: @@
 <td style="text-align:center; font-family:Verdana; font-weight:700;">
-[[Team:USC/Notebook/Brainstorming|Week 7]]
+[[Team:USC/Notebook/Week7|Week 7]]
 <br/>
 [[File:week7.jpg]]
@@ Line 96: / Line 96: @@
 <td style="text-align:center; font-family:Verdana; font-weight:700;">
-[[Team:USC/Notebook/Brainstorming|Week 8]]
+[[Team:USC/Notebook/Week8|Week 8]]
 <br/>
 [[File:week8.jpg]]
@@ Line 102: / Line 102: @@
 <td style="text-align:center; font-family:Verdana; font-weight:700;">
-[[Team:USC/Notebook/Brainstorming|Week 9]]
+[[Team:USC/Notebook/Week9|Week 9]]
 <br/>
 [[File:week9.jpg]]
@@ Line 108: / Line 108: @@
 <td style="text-align:center; font-family:Verdana; font-weight:700;">
-[[Team:USC/Notebook/Brainstorming|Week 10]]
+[[Team:USC/Notebook/Week10|Week 10]]
 <br/>
 [[File:week10.jpg]]
@@ Line 114: / Line 114: @@
 <td style="text-align:center; font-family:Verdana; font-weight:700;">
-[[Team:USC/Notebook/Brainstorming|Week 11]]
+[[Team:USC/Notebook/Week11|Week 11]]
 <br/>
 [[File:week11.jpg]]
@@ Line 120: / Line 120: @@
 <td style="text-align:center; font-family:Verdana; font-weight:700;">
-[[Team:USC/Notebook/Brainstorming|Week 12]]
+[[Team:USC/Notebook/Week12|Week 12]]
 <br/>
 [[File:week12.jpg]]
@@ Line 126: / Line 126: @@
 <td style="text-align:center; font-family:Verdana; font-weight:700;">
-[[Team:USC/Notebook/Brainstorming|Week 13]]
+[[Team:USC/Notebook/Week13|Week 13]]
 <br/>
 [[File:week13.jpg]]