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Team:Washington/Magnetosomes/Future
From 2011.igem.org
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<center><big><big><big><big>'''iGEM Toolkits: Future Directions'''</big></big></big></big></center><br><br> | <center><big><big><big><big>'''iGEM Toolkits: Future Directions'''</big></big></big></big></center><br><br> | ||
[[File:Washington_iGEM2011_futuretoolkit.png|300px|center]] | [[File:Washington_iGEM2011_futuretoolkit.png|300px|center]] | ||
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| + | Even though the end of iGEM 2011 is near, there is still a lot we would like to accomplish. By providing these toolkits, we hope to encourage future iGEM teams to experiment with Gibson cloning to help create large-scale constructs like the genome of Magnetotactic Bacteria. | ||
=='''Gibson Assembly Toolkit'''== | =='''Gibson Assembly Toolkit'''== | ||
| + | 1. '''Remove XhoI site from pGA3K3 vector''': | ||
| - | + | 2. '''Remove homologous regions from pGA4A5, pGA4C5 vectors''': these homologous regions within the backbone are self-complementary and can bind to one another, circularizing the backbone without an insert. This could potentially reduce the backbones efficiency and should be eliminated. | |
| - | + | ||
| - | 2. Remove homologous regions from pGA4A5, pGA4C5 vectors | + | |
| - | + | ||
| - | + | ||
| + | 3. Finally, we would like to continue adding more BioBrick compatible Gibson Vectors to help expand their popularity within the iGEM community. | ||
| - | + | =='''Magnetosome Toolkit'''== | |
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| - | + | ||
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| - | + | There is still much work to be done before we can achieve our goal of making '''Magnetocoli'''; however, we feel our toolkit provides a good foundation for future iGEM teams to experiment | |
| - | + | <br/> | |
| - | <br/> | + | |
*Express the rest of the gene in the R5 region of MAI in E.coli | *Express the rest of the gene in the R5 region of MAI in E.coli | ||
*Investigate other regions (R1-R14) of the Magnetosome Island | *Investigate other regions (R1-R14) of the Magnetosome Island | ||
Revision as of 00:19, 29 September 2011
Even though the end of iGEM 2011 is near, there is still a lot we would like to accomplish. By providing these toolkits, we hope to encourage future iGEM teams to experiment with Gibson cloning to help create large-scale constructs like the genome of Magnetotactic Bacteria.
Gibson Assembly Toolkit
1. Remove XhoI site from pGA3K3 vector:
2. Remove homologous regions from pGA4A5, pGA4C5 vectors: these homologous regions within the backbone are self-complementary and can bind to one another, circularizing the backbone without an insert. This could potentially reduce the backbones efficiency and should be eliminated.
3. Finally, we would like to continue adding more BioBrick compatible Gibson Vectors to help expand their popularity within the iGEM community.
Magnetosome Toolkit
There is still much work to be done before we can achieve our goal of making Magnetocoli; however, we feel our toolkit provides a good foundation for future iGEM teams to experiment
- Express the rest of the gene in the R5 region of MAI in E.coli
- Investigate other regions (R1-R14) of the Magnetosome Island
- Co-express the genes and study their interaction
- Build the scaffold structure in E.coli
- Express the full assembly in E.coli
- Develop assay for the magnet formation
- Determine the optimal cell growth condition......
In addition, the ability to produce and control uniform, nano-sized magnetic particles is attractive in areas such as medical imaging and nano-electronics where scientists and engineers are actively seeking innovative solutions for advancements in size and accuracy. Thus, if we are able to produce magnets in an organism that are thoroughly understood in a controlled manner, and be able to extract the magnets from them, this indeed is going to be very useful for a lot of areas.
