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Team:EPF-Lausanne/Todo
From 2011.igem.org
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*Adapt design of "chemostat" chip for e-coli | *Adapt design of "chemostat" chip for e-coli | ||
| - | == Preparing the parts == | + | == <s>Preparing the parts</s> == |
* <s>Sequence the lysis cassette</s> | * <s>Sequence the lysis cassette</s> | ||
| - | * Double-check lysis cassette sequence | + | * <s>Double-check lysis cassette sequence</s> |
| + | |||
| + | All the parts are verified, we can now assemble them! | ||
== Assembly == | == Assembly == | ||
Revision as of 15:29, 21 June 2011
Todo
Contents |
Microfluidic Chips
- Continue alignment training
- Run experiments on "chemostat" chip to check design
- Adapt design of "chemostat" chip for e-coli
Preparing the parts
-
Sequence the lysis cassette -
Double-check lysis cassette sequence
All the parts are verified, we can now assemble them!
Assembly
-
Research plasmid backbones. Is there one that already contains Tet-repressed LacI or GFP? - Design Gibson primers to assemble the three different plasmids.
- Determine which sequence on LacI-plasmid and Lysis-plasmid should be used to create the "mega-plasmid".
Wiki
Protocols
-
Describe cell cultures in miniprep protocol - Write a new protocol!
- Upload "chemostat" protocols
General
- Write-up team presentation
- Upload our initial research about transcription factors
Clean room
- Order lab notebook
-
Order storage box
Calendar
