Revision as of 03:10, 22 September 2011

In Vivo & In Vitro Outline

In vitro Main | Why TetR? | Mutant TetRs | MITOMI Data | In-vivo & In-vitro outline

For some of the TetR mutants, we managed to have both an in vitro and in vivo characterization. The results are available in the table below.

Mutant	Biobrick number	Affinity compared to WT - in vivo	Affinity compared to WT - MITOMI	ATC repression
V36F	BBa_K613013	same	same	altered
V36F W43S	BBa_K613014	same	not tested	altered
E37A W43S T141A	BBa_K613015	same		altered
P39K	BBa_K613016	no affinity		normal
Y42F K108E	BBa_K613018	reduced		normal
P39Q Y42M	BBa_K613019	no affinity	not tested	normal

The MITOMI results can be found here. The in vivo characterizations are on this page.

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