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Team:UCL London/Research/Stresslights/Experiments
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Revision as of 01:59, 22 September 2011
Modifying Oximeter in the lab
Aim
To modify and improve the oximeter device (BBa_K239011) using modified nark promoter (BBa_K239006) submitted by 2009 UCL iGEM team. Changes included replacing the normal GFP (green fluorescent protein) with an enhanced YFP (yellow fluorescent protein), which has an LVA tag added to its C terminal.
Parts used
Part Name |
Parts ID |
Parts Description |
Length (bp) |
Plasmid Backbone |
mNARK promoter |
promoter sensitive to hypoxia |
89 |
on pUC57 (2710 bp) - ampicillin resistance |
|
RBS |
ribosome binding site |
12 |
on pSB1A2 (2079 bp) - ampicillin resistance |
|
YFP.LVA |
enhanced yellow fluorescent protein with LVA tag |
759 |
on pSB1A2 (2079 bp) |
|
Terminator |
double terminator (B0010-B0012) |
129 |
on pSB1A2 (2079 bp) |
|
n/a |
standard plasmid backbone (kanamycin resistance) |
2204 |
n/a |
|
n/a |
standard plasmid backbone (tetracycline resistance) |
2463 |
n/a |
|
n/a |
standard plasmid backbone (chloramphenicol resistance) |
2070 |
n/a |
