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Team:Paris Bettencourt/Experiments/Methodologies/K090403J
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| - | == | + | ==Integration== |
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| + | After having used a replicative plasmid (pHM3), we tried to use a Bacillus subtilis integration plasmid. For that, we used and plan to continue the construction of the B.subtilis integration vector pSBINT1 built by the 2008 Cambridge iGEM team. | ||
{| border="1" class="wikitable" style="text-align: center;" | {| border="1" class="wikitable" style="text-align: center;" | ||
|+ Bacillus subtilis integration vector | |+ Bacillus subtilis integration vector | ||
|- | |- | ||
| - | |[[File:PBSINT1_final.png|900px|thumb|center| | + | |[[File:PBSINT1_final.png|900px|thumb|center|]] |
|- | |- | ||
|} | |} | ||
| + | At first, we managed to clone each of our parts in the 5' integration sequence for the AmyE locus of B.subtilis (K143001). After, we want to clone them into the integrative plasmid. | ||
<html> | <html> | ||
Revision as of 01:49, 22 September 2011
Integration
After having used a replicative plasmid (pHM3), we tried to use a Bacillus subtilis integration plasmid. For that, we used and plan to continue the construction of the B.subtilis integration vector pSBINT1 built by the 2008 Cambridge iGEM team.
 |
At first, we managed to clone each of our parts in the 5' integration sequence for the AmyE locus of B.subtilis (K143001). After, we want to clone them into the integrative plasmid.
