Team:EPF-Lausanne/Our Project/TetR mutants

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In order to generate new libraries of transcription factors, we first need a convenient way to induce mutations at specific positions, to mutate the amino acids involved in DNA binding. Then, once having generated mutants, it is needed to have a precise and high-throughput characterization method - these two requirements being present in the microfluidics MITOMI device that we used.
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Characterizing a lot of different mutants will allow to get a better understanding on the the factors influencing the specificity and affinity of a transcription factor. Once with these parameters in hand, we will be able to fine-tune the characteristics of the newly produced TFs.
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The new transcription factors mutants characterized in vitro then need to be tested in vitro. This last step is explained in the " ''in vivo'' characterization section".
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Revision as of 02:15, 22 September 2011