Team:Glasgow/Results/MCS
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<h2>Aims</h2> | <h2>Aims</h2> | ||
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- To develop a more efficient method for testing large numbers of biobricks<br/> | - To develop a more efficient method for testing large numbers of biobricks<br/> | ||
Latest revision as of 03:54, 22 September 2011
![](http://farm7.static.flickr.com/6122/5958857609_b9df31d718_b.jpg)
Aims
Back to Results
- To develop a more efficient method for testing large numbers of biobricksMethod
The restriction enzymes we chose to include in our Multiple Cloning Site are XhoI, BamHI, SalI, BglI and HindIII.![](https://static.igem.org/mediawiki/2011/0/00/Glasgowmcsonly.png)
We designed primers and had the Multiple Cloning Site biobrick synthesized. These primers are below:
Forward Primer | 5'-AATTCGCGGCCGCTTCTAGAGGGTACCGGCGCCCTCGAGGGTCCAGCTTTACTAGAAAAAAAAACCCCGCCCCTGACAGGGCGGGGTTTTT-3' |
Reverse Primer |
5'-GCGGCCGCTACTAGTAAAAAAAAACCCCGCCCTGTCAGGGGCGGGGTTTTTTTTTCTCTAGTAAAGCTTGGATCCCTCGAGGGCGCCGGTACC CTCTAGAAGCGGCCGCG-3' |
The part is still awaiting synthesis.
You can visit the Multiple Cloning Site Part Page here