Team:Paris Bettencourt/Experiments/Methodologies

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<p>Creating nanotubes under the microscope need a lot of tuning. In these pages, we recapitulates the methodologies we use to prepare the microscope slides with the cell properly prepared, and how we have builded the microfluidic chips in wich we trapped B. subtilis strains</p>
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<p><em><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/Microscopy">Preparing the microscope slides:</a></em> Visit this page if you want to learn how to grow B. <i>subtilis</i> strains in condition you can observe the nanotube.<p>
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<p><em><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/Microfluidic">See you new microfluidic chip:</a></em> We suceeded in creating a <em>completely new</em> microfluidic chip, that acts as a chemostat, with the apropriate environment to observe the nanotubes.<p>
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We have modified a microfluidic system from Jeff Hasty group in UCSD, in which we can grow bacterial cells continuously for a long time in a single layer. We called it the <b><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/Methodologies/Microchemostat_HastyJ">Micro-chemostat</a></b>. Compared to the usual microcolony-on-agar-pad method in the paper, this system will be able to maintain exponential growth of the bacterial cells in close contact with each other for a long time without exhausting the nutrient nor form double layers. We hope by extending the imaging time, we'll be able to observe the more diffusion-through-nanotube events in a single experiment.
We have modified a microfluidic system from Jeff Hasty group in UCSD, in which we can grow bacterial cells continuously for a long time in a single layer. We called it the <b><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/Methodologies/Microchemostat_HastyJ">Micro-chemostat</a></b>. Compared to the usual microcolony-on-agar-pad method in the paper, this system will be able to maintain exponential growth of the bacterial cells in close contact with each other for a long time without exhausting the nutrient nor form double layers. We hope by extending the imaging time, we'll be able to observe the more diffusion-through-nanotube events in a single experiment.
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Revision as of 00:00, 22 September 2011

Team IGEM Paris 2011

Methodologies

Creating nanotubes under the microscope need a lot of tuning. In these pages, we recapitulates the methodologies we use to prepare the microscope slides with the cell properly prepared, and how we have builded the microfluidic chips in wich we trapped B. subtilis strains

Preparing the microscope slides: Visit this page if you want to learn how to grow B. subtilis strains in condition you can observe the nanotube.

See you new microfluidic chip: We suceeded in creating a completely new microfluidic chip, that acts as a chemostat, with the apropriate environment to observe the nanotubes.
We have modified a microfluidic system from Jeff Hasty group in UCSD, in which we can grow bacterial cells continuously for a long time in a single layer. We called it the Micro-chemostat. Compared to the usual microcolony-on-agar-pad method in the paper, this system will be able to maintain exponential growth of the bacterial cells in close contact with each other for a long time without exhausting the nutrient nor form double layers. We hope by extending the imaging time, we'll be able to observe the more diffusion-through-nanotube events in a single experiment.

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