Team:Paris Bettencourt/Experiments/Methodologies

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<h2>Microfluidic system </h2>
<h2>Microfluidic system </h2>
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<b><a href="https://2011.igem.org/Team:Paris_Bettencourt/T7_diffusion">T7 RNA polymerase diffusion</a></b>
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We have modified a microfluidic system from Jeff Hasty group in UCSD, in which we can grow bacterial cells continuously for a long time in a single layer. We called it the <b><a href="https://2011.igem.org/Team:Paris_Bettencourt/Experiments/Methodologies/Microchemostat_HastyJ">Micro-chemostat</a></b>. Compared to the usual microcolony-on-agar-pad method in the paper, this system will be able to maintain exponential growth of the bacterial cells in close contact with each other for a long time without exhausting the nutrient nor form double layers. We hope by extending the imaging time, we'll be able to observe the more diffusion-through-nanotube events in a single experiment.
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Revision as of 23:58, 21 September 2011

Team IGEM Paris 2011

Microfluidic system

We have modified a microfluidic system from Jeff Hasty group in UCSD, in which we can grow bacterial cells continuously for a long time in a single layer. We called it the Micro-chemostat. Compared to the usual microcolony-on-agar-pad method in the paper, this system will be able to maintain exponential growth of the bacterial cells in close contact with each other for a long time without exhausting the nutrient nor form double layers. We hope by extending the imaging time, we'll be able to observe the more diffusion-through-nanotube events in a single experiment.

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