Team:Glasgow/Results/MCS
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We designed primers and had the Multiple Cloning Site biobrick synthesized. These primers are below:<br/><Br/> | We designed primers and had the Multiple Cloning Site biobrick synthesized. These primers are below:<br/><Br/> | ||
- | <table border="1"> | + | <table border="1" width="890px"> |
<tr> | <tr> | ||
<td>Forward Primer</td> | <td>Forward Primer</td> | ||
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<tr> | <tr> | ||
<td>Reverse Primer</td> | <td>Reverse Primer</td> | ||
- | <td>5'- | + | <td> |
+ | 5'-GCGGCCGCTACTAGTAAAAAAAAACCCCGCCCTGTCAGGGGCGGGGTTTTTTTTTCTCTAGTAAAGCTTGGATCCCTCGAGGGCGCCGGTACC<br/> | ||
+ | CTCTAGAAGCGGCCGCG-3' | ||
</tr> | </tr> | ||
</table> | </table> |
Revision as of 23:26, 21 September 2011
![](http://farm7.static.flickr.com/6122/5958857609_b9df31d718_b.jpg)
Aims
- To produce an easier method for testing large numbers of biobricks- To reduce the overall number of ligations needed to do this
Method
The restriction enzymes we chose to include in our Multiple Cloning Site are XhoI, BamHI, SalI, BglI and HindIII.![](https://static.igem.org/mediawiki/2011/0/00/Glasgowmcsonly.png)
We designed primers and had the Multiple Cloning Site biobrick synthesized. These primers are below:
Forward Primer | 5'-AATTCGCGGCCGCTTCTAGAGGGTACCGGCGCCCTCGAGGGTCCAGCTTTACTAGAAAAAAAAACCCCGCCCCTGACAGGGCGGGGTTTTT-3' |
Reverse Primer |
5'-GCGGCCGCTACTAGTAAAAAAAAACCCCGCCCTGTCAGGGGCGGGGTTTTTTTTTCTCTAGTAAAGCTTGGATCCCTCGAGGGCGCCGGTACC CTCTAGAAGCGGCCGCG-3' |
The part is still awaiting synthesis.
You can visit the Multiple Cloning Site Part Page here