Team:Groningen/project characterisation mu lasr

From 2011.igem.org

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Our second memory unit consists of a LasR gene(Bba_C0179) driven the LasB promoter(Bba_R0079). LasR only activates transcription in the presence of HSL, this was added to the medium in a concentration of 100nM.  
Our second memory unit consists of a LasR gene(Bba_C0179) driven the LasB promoter(Bba_R0079). LasR only activates transcription in the presence of HSL, this was added to the medium in a concentration of 100nM.  
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==Leakiness of the LasR autoinducing loops==
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==Leakage of the LasR autoinducing loops==
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To estimate the autoactivation of the LasR loop through a leaky promoter we measured fluorescence of cells carrying the following constructs:
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[[File:lasR4]]
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[[File:lasR6]]
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{| class="wikitable"
{| class="wikitable"
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| RBS 1 || <gallery>File:lasr12.png| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K607034 BBa_K607034]</gallery> || <gallery>File:lasr8.png| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K607030 BBa_K607030]</gallery> || <gallery>File:lasR4.png| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K607026 BBa_K607026]</gallery>
| RBS 1 || <gallery>File:lasr12.png| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K607034 BBa_K607034]</gallery> || <gallery>File:lasr8.png| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K607030 BBa_K607030]</gallery> || <gallery>File:lasR4.png| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K607026 BBa_K607026]</gallery>
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|colspan="4"| '''Figure 4.''' Flow cytometer data taken at different time-points during exponential growth of cells carrying different cI autoinducing loops.
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|colspan="4"| '''Figure 1.''' Flow cytometer data obtained at different time-points during exponential growth of cells carrying different LasR autoinducing loops in presence of 100 nM HSL.
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{{FooterGroningen2011}}

Revision as of 23:17, 21 September 2011


LasR Autoinducing Loops

Our second memory unit consists of a LasR gene(Bba_C0179) driven the LasB promoter(Bba_R0079). LasR only activates transcription in the presence of HSL, this was added to the medium in a concentration of 100nM.

Leakage of the LasR autoinducing loops

To estimate the autoactivation of the LasR loop through a leaky promoter we measured fluorescence of cells carrying the following constructs:

no-tag DAS LVA
RBS 0,07

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607031 BBa_K607031]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607027 BBa_K607027]

LasR1.png

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607023 BBa_K607023]

Legenda.png
RBS 0,3

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607032 BBa_K607032]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607028 BBa_K607028]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607024 BBa_K607024]

RBS 0,6

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607033 BBa_K607033]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607029 BBa_K607029]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607025 BBa_K607025]

RBS 1

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607034 BBa_K607034]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607030 BBa_K607030]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607026 BBa_K607026]

Figure 1. Flow cytometer data obtained at different time-points during exponential growth of cells carrying different LasR autoinducing loops in presence of 100 nM HSL.




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