Revision as of 22:37, 21 September 2011

muTetRs

In vitro Main | Why TetR? | Mutant TetRs | MITOMI Data | In-vivo & In-vitro outline

First we chose the site directed mutagenesis approach to make the mutants of TetR but that strategy did not work well

TetR variants	Mutagenesis	Promoter	Cterminal	DNA form	Expression	MITOMI vs 1-off	Biobrick	Sequenced	Sent to registry
V36F	site directed	SP6	eGFP	plasmid	worked well	X	BBa_K613013
V36FW43S	PCR-induced	T7	His-tag	linear	(ins C-term), ITT tested		BBa_K613014
E37AW43ST141A	PCR-induced	T7	His-tag	linear	ITT tested	X	BBa_K613015	X	X
P39K	PCR-induced	T7	His-tag	template	ITT tested, worked	X	BBa_K613016	X	X
Y42F	PCR-induced	T7	His-tag	linear	ITT tested	X	BBa_K613017	X	X
Y42FK108E	PCR-induced	T7	His-tag	linear	ITT tested		BBa_K613018	X	X
P39QY42M	site directed	SP6	eGFP	plasmid	worked well	X	BBa_K613019
P39QY42ML197S	PCR-induced	T7	His-tag	linear	ITT tested		BBa_K613020
P39QY42ML52P	PCR-induced	T7	His-tag	linear	ITT tested		BBa_K613021
E37AP39K	site directed	T7	His-tag	linear			BBa_K613022	X
E37AP39QY42F	site directed	T7	His-tag	linear			BBa_K613023	X
WT	none	T7	His&GFP	both

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	==How==		==How==

-	First we chose the site directed mutagenesis to make the mutants of TetR but that strategy <html> <a href="https://2011.igem.org/Team:EPF-Lausanne/Notebook/July2011#Tuesday.2C_12_July_2011"> did not work well </a> </html>	+	First we chose the site directed mutagenesis approach to make the mutants of TetR but that strategy <html> <a href="https://2011.igem.org/Team:EPF-Lausanne/Notebook/July2011#Tuesday.2C_12_July_2011"> did not work well </a> </html>