Team:Groningen/project characterisation mu lasr

From 2011.igem.org

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| RBS 0,3 || <gallery>File:lasR10.png| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K607032 BBa_K607032]</gallery> || <gallery>File:lasR6.png| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K607028 BBa_K607028]</gallery> || <gallery>File:lasR2.png| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K607024 BBa_K607024]</gallery>
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| RBS 0,6 || <gallery> File:lasR11.png|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607033 BBa_K607033] </gallery>
| RBS 0,6 || <gallery> File:lasR11.png|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607033 BBa_K607033] </gallery>

Revision as of 22:32, 21 September 2011


LasR Autoinducing Loops

Our second memory unit consists of a LasR gene(Bba_C0179) driven the LasB promoter(Bba_R0079). LasR only activates transcription in the presence of HSL, this was added to the medium in a concentration of 100nM.

Lasr2.png

Leakiness of the LasR autoinducing loops

File:LasR1.png LasR2.png LasR3.png LasR4.png LasR5.png LasR6.png File:LasR7.png LasR8.png LasR9.png LasR10.png LasR11.png LasR12.png


no-tag DAS LVA
RBS 0,07 Legenda.png
RBS 0,3
RBS 0,6
RBS 1
Figure 4. Flow cytometer data taken at different time-points during exponential growth of cells carrying different cI autoinducing loops.