Team:Glasgow/Results/MCS
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<td>Reverse Primer</td> | <td>Reverse Primer</td> | ||
- | <td>5'-GCGGCCGCTACTAGTAAAAAAAAACCCCGCCCTGTCAGGGGCGGGG | + | <td>5'-GCGGCCGCTACTAGTAAAAAAAAACCCCGCCCTGTCAGGGGCGGGG TTTTTTTTTCTCTAGTAAAGCTTGGATCCCTCGAGGGCGCCGGTACCCTCTAGAAGCGGCCGCG-3'</td> |
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</table> | </table> |
Revision as of 22:06, 21 September 2011
![](http://farm7.static.flickr.com/6122/5958857609_b9df31d718_b.jpg)
Aims
- To produce an easier method for testing large numbers of biobricks- To reduce the overall number of ligations needed to do this
Method
The restriction enzymes we chose to include in our Multiple Cloning Site are XhoI, BamHI, SalI, BglI and HindIII.![](https://static.igem.org/mediawiki/2011/0/00/Glasgowmcsonly.png)
We designed primers and had the Multiple Cloning Site biobrick synthesized. These primers are below:
Forward Primer | 5'-aattcgcggccgcttctagagggtaccggcgccctcgagggatcca agcttTactagagaaaaaaaaaccccgcccctgacagggcggggttttt ttttactagtagcggccgctgca-3' |
Reverse Primer | 5'-GCGGCCGCTACTAGTAAAAAAAAACCCCGCCCTGTCAGGGGCGGGG TTTTTTTTTCTCTAGTAAAGCTTGGATCCCTCGAGGGCGCCGGTACCCTCTAGAAGCGGCCGCG-3' |