"
Team:Glasgow/Results/MCS
From 2011.igem.org
(Difference between revisions)
HannahRalph (Talk | contribs) |
HannahRalph (Talk | contribs) |
||
| Line 11: | Line 11: | ||
<br/><Br/> | <br/><Br/> | ||
We designed primers and had the Multiple Cloning Site biobrick synthesized. These primers are below:<br/><Br/> | We designed primers and had the Multiple Cloning Site biobrick synthesized. These primers are below:<br/><Br/> | ||
| - | + | <table border="1"> | |
| - | < | + | <tr> |
| - | + | <td>Forward Primer</td> | |
| + | <td> | ||
5'-aattcgcggccgcttctagagggtaccggcgccctcgagggatcca | 5'-aattcgcggccgcttctagagggtaccggcgccctcgagggatcca | ||
agcttTactagagaaaaaaaaaccccgcccctgacagggcggggttttt | agcttTactagagaaaaaaaaaccccgcccctgacagggcggggttttt | ||
| - | ttttactagtagcggccgctgca-3' | + | ttttactagtagcggccgctgca-3'</td> |
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Reverse Primer</td> | ||
| + | <td>5'-GCGGCCGCTACTAGTAAAAAAAAACCCCGCCCTGTCAGGGGCGGGG | ||
| - | + | TTTTTTTTTCTCTAGTAAAGCTTGGATCCCTCGAGGGCGCCGGTACCCT | |
| - | < | + | CTAGAAGCGGCCGCG-3'</td> |
| + | </tr> | ||
| + | </table> | ||
| - | |||
| - | |||
| - | |||
| - | + | <h2>Results</h2> | |
Revision as of 22:04, 21 September 2011
Aims
- To produce an easier method for testing large numbers of biobricks- To reduce the overall number of ligations needed to do this
Method
The restriction enzymes we chose to include in our Multiple Cloning Site are XhoI, BamHI, SalI, BglI and HindIII.
We designed primers and had the Multiple Cloning Site biobrick synthesized. These primers are below:
| Forward Primer | 5'-aattcgcggccgcttctagagggtaccggcgccctcgagggatcca agcttTactagagaaaaaaaaaccccgcccctgacagggcggggttttt ttttactagtagcggccgctgca-3' |
| Reverse Primer | 5'-GCGGCCGCTACTAGTAAAAAAAAACCCCGCCCTGTCAGGGGCGGGG TTTTTTTTTCTCTAGTAAAGCTTGGATCCCTCGAGGGCGCCGGTACCCT CTAGAAGCGGCCGCG-3' |
