"
Team:Valencia/Notebook/Week12
From 2011.igem.org
(Difference between revisions)
| Line 262: | Line 262: | ||
<div class=col_center_top><b>WEEK 12</b></div><!-- fin clase col_left_top--> | <div class=col_center_top><b>WEEK 12</b></div><!-- fin clase col_left_top--> | ||
<div class=col_center_center> | <div class=col_center_center> | ||
| - | <b>Hardware</b> | + | <b>Hardware:</b> |
<br><br> | <br><br> | ||
| - | <b>pH-stat</b> | + | <b>pH-stat:</b> There was a large increase in the concentration of Synechocystis, especially in flasks 5 and 6, but also contamination from other microorganisms. |
| + | <p>We started two new 80 ml cultures to which 2 ml of fertilizer to each one was added. They finally began to develop favourably, increasing their concentration and also we could see a vast majority of growing cells against the cells which are not reproducing.</p> | ||
| + | <p>Once we obtained and established the culture under laboratory conditions, we measured the change in pH. Since the 24 hour light had caused serious damage to the culture, we measured the pH changes that occurred each hour in photoperiods of 4 hours of light and 4 of darkness.</p> | ||
| + | |||
<br><br> | <br><br> | ||
| - | <b>Colicin production</b> We start growing the E.coli with microcin C51 in LB medium with ampicilin. And we perform a viable count and a plasmid extraction too.<br> | + | <b>Colicin production:</b> We start growing the E.coli with microcin C51 in LB medium with ampicilin. And we perform a viable count and a plasmid extraction too.<br> |
</div><!-- fin clase col_left_top--> | </div><!-- fin clase col_left_top--> | ||
Revision as of 21:07, 21 September 2011
WEEK 12
Hardware:
pH-stat: There was a large increase in the concentration of Synechocystis, especially in flasks 5 and 6, but also contamination from other microorganisms.
Colicin production: We start growing the E.coli with microcin C51 in LB medium with ampicilin. And we perform a viable count and a plasmid extraction too.
pH-stat: There was a large increase in the concentration of Synechocystis, especially in flasks 5 and 6, but also contamination from other microorganisms.
We started two new 80 ml cultures to which 2 ml of fertilizer to each one was added. They finally began to develop favourably, increasing their concentration and also we could see a vast majority of growing cells against the cells which are not reproducing.
Once we obtained and established the culture under laboratory conditions, we measured the change in pH. Since the 24 hour light had caused serious damage to the culture, we measured the pH changes that occurred each hour in photoperiods of 4 hours of light and 4 of darkness.
Colicin production: We start growing the E.coli with microcin C51 in LB medium with ampicilin. And we perform a viable count and a plasmid extraction too.
