Revision as of 21:43, 21 September 2011

muTetRs

In vitro Main | Why TetR? | Mutant TetRs | MITOMI Data | In-vivo & In-vitro outline

First we chosed the site directed mutagenesis to make the mutants of TetR

TetR variants	Mutagenesis	Promoter	Cterminal	DNA form	Expression	MITOMI vs 1-off	Biobrick	Sequenced	Sent to registry
V36F	site directed	SP6	eGFP	plasmid	worked well	X	BBa_K613013
V36FW43S	PCR-induced	T7	His-tag	linear	(ins C-term), ITT tested		BBa_K613014
E37AW43ST141A	PCR-induced	T7	His-tag	linear	ITT tested	X	BBa_K613015	X	X
P39K	PCR-induced	T7	His-tag	template	ITT tested, worked	X	BBa_K613016	X	X
Y42F	PCR-induced	T7	His-tag	linear	ITT tested	X	BBa_K613017	X	X
Y42FK108E	PCR-induced	T7	His-tag	linear	ITT tested		BBa_K613018	X	X
P39QY42M	site directed	SP6	eGFP	plasmid	worked well	X	BBa_K613019
P39QY42ML197S	PCR-induced	T7	His-tag	linear	ITT tested		BBa_K613020
P39QY42ML52P	PCR-induced	T7	His-tag	linear	ITT tested		BBa_K613021
E37AP39K	site directed	T7	His-tag	linear			BBa_K613022	X
E37AP39QY42F	site directed	T7	His-tag	linear			BBa_K613023	X
WT	none	T7	His&GFP	both

@@ Line 4: / Line 4: @@
 ==How==
+First we chosed the site directed mutagenesis to make the mutants of TetR
+[[File:PCR_sketch.png]]
 ==What==