Team:Imperial College London/Project Gene Future

From 2011.igem.org

(Difference between revisions)
Line 5: Line 5:
<body>
<body>
<h1>Future Work</h1>
<h1>Future Work</h1>
-
<p>1. Alternative to antibiotic resistance </p>
+
 
 +
<p>To carry on the work on the Gene Guard module, there are a number of steps we would take in the immediate future and others that form part of our long term plan.
 +
 
 +
<h2>Short-term plans</h2>
 +
<p>1. 16s rRNA of the bacteria grown up from sterile and non-sterile soil will be sequenced to determine the bacterial species present.
<p>2. Replicates of the soil experiment will be analysed for expression of sfGFP.</p>
<p>2. Replicates of the soil experiment will be analysed for expression of sfGFP.</p>
-
<p>2. 16s rRNA of the bacteria grown up from sterile and non-sterile soil will be sequenced to determine the bacterial species present.  
+
<p>3.
 +
 
 +
 
 +
<h2>Long-term plans</h2>
 +
 
 +
<p>1.
 +
<p>2.
 +
<p>3. Alternative to antibiotic resistance </p>
</body>
</body>
</html>
</html>

Revision as of 20:02, 21 September 2011




Module 3: Gene Guard

Containment is a serious issue concerning the release of genetically modified organisms (GMOs) into the environment. To prevent horizontal gene transfer of the genes we are expressing in our chassis, we have developed a system based on the genes encoding holin, anti-holin and endolysin. We are engineering anti-holin into the genome of our chassis, where it acts as an anti-toxin, and holin and endolysin on plasmid DNA. In the event of horizontal gene transfer with a soil bacterium, holin and endolysin will be transferred without anti-holin, rendering the recipient cell non-viable and effectively containing the Auxin Xpress and Phyto-Route genes in our chassis.




Future Work

To carry on the work on the Gene Guard module, there are a number of steps we would take in the immediate future and others that form part of our long term plan.

Short-term plans

1. 16s rRNA of the bacteria grown up from sterile and non-sterile soil will be sequenced to determine the bacterial species present.

2. Replicates of the soil experiment will be analysed for expression of sfGFP.

3.

Long-term plans

1.

2.

3. Alternative to antibiotic resistance